重组Anti-NDUFAF1抗体[EPR2796] (ab79826)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2796] to NDUFAF1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NDUFAF1抗体[EPR2796]
参阅全部 NDUFAF1 一抗 -
描述
兔单克隆抗体[EPR2796] to NDUFAF1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human NDUFAF1 aa 300-400 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: HeLa, Jurkat and heart lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2796 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79826于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/250.
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WB |
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa).
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ICC/IF |
1/100 - 1/500.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/250. |
WB
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the CIA30 family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 51103 Human
- Entrez Gene: 69702 Mouse
- Entrez Gene: 296086 Rat
- Omim: 606934 Human
- SwissProt: Q9Y375 Human
- SwissProt: Q9CWX2 Mouse
- Unigene: 106529 Human
- Unigene: 5390 Mouse
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别名
- CGI 65 antibody
- CGI65 antibody
- CIA30 antibody
see all
图片
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All lanes : Anti-NDUFAF1 antibody [EPR2796] (ab79826) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling NDUFAF1 with purified 79826 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NDUFAF1 (red) with ab79826 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 ab79826.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells using 1/100 ab79826.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab79826 被引用在 3 文献中.
- Wongkitikamjorn W et al. Gestational Intermittent Hypoxia Induces Mitochondrial Impairment in the Geniohyoid Muscle of Offspring Rats. Cureus 14:e25088 (2022). PubMed: 35600069
- Miwa S et al. Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. Nat Commun 5:3837 (2014). WB ; Mouse . PubMed: 24815183
- Ekman M et al. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest 94:557-68 (2014). PubMed: 24589856