Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (1%BSA+0.2%Tween-20)
Dilution
1/1000
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Donkey anti Mouse IgM - Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
This antibody specifically recognizes NCX1 in cytoplasm and cell membrane of cardiomyocytes. Negative control without primary antibody didn't have any signal when using the same imaging settings as experiment group.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 15 2020