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Signal Transduction Signaling Pathway Calcium Signaling Calcium Channels

Anti-NCX1抗体[C2C12] (ab2869)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (23)

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Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
  • Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)

Key features and details

  • Mouse monoclonal [C2C12] to NCX1
  • Suitable for: IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

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概述

  • 产品名称

    Anti-NCX1抗体[C2C12]
    参阅全部 NCX1 一抗
  • 描述

    小鼠单克隆抗体[C2C12] to NCX1
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Human
    IHC-P
    Human
    See all applications and species data
  • 免疫原

    Full length native protein (purified) corresponding to Dog NCX1. Purified from canine cardiac sodium/calcium exchanger.

  • 表位

    This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane.
  • 阳性对照

    • IHC-P: Normal human kidney tissue. IHC-Fr: Normal human kidney tissue
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Primary antibody说明

    The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells.
  • 克隆

    单克隆
  • 克隆编号

    C2C12
  • 同种型

    IgM
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (ab47827)
    • Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
IHC-Fr
Human
IHC-P
Human
All applications
Mouse
Rat
Rabbit
Guinea pig
Dog
Pig
应用 Ab评论 说明
IHC-P (2)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr (1)
Use a concentration of 1 µg/ml.

PubMed: 21408028

Flow Cyt
1/20 - 1/100.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

说明
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use a concentration of 1 µg/ml.

PubMed: 21408028

Flow Cyt
1/20 - 1/100.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.
  • 组织特异性

    Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung.
  • 序列相似性

    Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily.
    Contains 2 Calx-beta domains.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P32418 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6546 Human
    • Entrez Gene: 20541 Mouse
    • Entrez Gene: 397365 Pig
    • Entrez Gene: 100328570 Rabbit
    • Entrez Gene: 29715 Rat
    • Omim: 182305 Human
    • SwissProt: P23685 Dog
    • SwissProt: P48766 Guinea pig
    • SwissProt: P32418 Human
    • SwissProt: P70414 Mouse
    • SwissProt: Q01728 Rat
    • Unigene: 31961 Human
    • Unigene: 468274 Human
    • Unigene: 728911 Human
    • Unigene: 265834 Mouse
    • Unigene: 118972 Rat
    see all
  • 别名

    • CNC antibody
    • DKFZp779F0871 antibody
    • MGC119581 antibody
    • Na(+)/Ca(2+)-exchange protein 1 antibody
    • Na+/Ca2+ exchange protein 1 antibody
    • Na+/Ca2+ exchanger antibody
    • NAC1_HUMAN antibody
    • NCX 1 antibody
    • NCX antibody
    • NCX1 antibody
    • SLC8A1 antibody
    • SLC8A1 protein antibody
    • Sodium Calcium Exchanger antibody
    • Sodium/calcium exchanger 1 antibody
    • Solute carrier family 8 (sodium/calcium exchanger) member 1 antibody
    • Solute carrier family 8 member 1 antibody
    see all

图片

  • Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (ab2869)

    IHC image of NCX1 staining in a section of frozen normal human kidney*. The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab2869 at 1µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), 1/1000)) (shown in green) and ab150080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594), 1/1000) (shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)

    IHC image of NCX1 staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2869, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)
    Flow Cytometry - Anti-NCX1 antibody [C2C12] (ab2869)

    Overlay histogram showing HEK293 cells stained with ab2869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2869, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCX1 antibody [C2C12] (ab2869)This image is courtesy of an anonymous Abreview
    ab2869 staining NCX1 in Human kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% normal goat serum in 1XPBS + 0.05% Tween20 for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate (pH 6.0) buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 25°C. Ab47827 (1/500) was used as the secondary antibody.

    See Abreview

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (23)

发表研究结果有使用 ab2869?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2869 被引用在 23 文献中.

  • Sun F  et al. Detrimental Effect of C-Reactive Protein on the Cardiometabolic Cells and Its Rectifying by Metabolic Surgery in Obese Diabetic Patients. Diabetes Metab Syndr Obes 13:1349-1358 (2020). PubMed: 32425567
  • Tian M  et al. NL1 expression level in Nrx1ß and the excitability of PV interneurons in mice with POCD. Exp Ther Med 17:3117-3123 (2019). PubMed: 30936983
  • Cheng PC  et al. The Na+/H+-Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca2+]i in the Suprachiasmatic Nucleus. Sci Rep 9:6430 (2019). PubMed: 31015514
  • Sahin K  et al. Effects of dietary supplementation of arginine-silicate-inositol complex on absorption and metabolism of calcium of laying hens. PLoS One 13:e0189329 (2018). WB ; Chicken . PubMed: 29360830
  • Cheng PC  et al. Differential regulation of nimodipine-sensitive and -insensitive Ca2+ influx by the Na+/Ca2+ exchanger and mitochondria in the rat suprachiasmatic nucleus neurons. J Biomed Sci 25:44 (2018). PubMed: 29788971
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Sep 15 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (coronary artery, heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6)
Permeabilization
No
Specification
coronary artery, heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 27 2017

Question

Our customer retested following your recommendation but the band still did not show in positive control (Hek293 cells).
She said that secondary antibody has been used for WB without any problem.
Please check attached file.

Best regards,

Read More

Abcam community

Verified customer

Asked on Jun 28 2012

Answer

Thank you and your customerfor taking the time totry the tipsand contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

The details your customerhas kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time your customerhas spent in the laboratory and understandtheir concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest your customerhas regrettably received a bad vial.

I apologise for the inconvenience and am pleased to offer your customera free of charge replacement (also possible with an alternative antibody) or credit note in compensation.

In addition, I can suggest your customer may like to consider the following suggestions for future experiments:

Thank you and your customerfor your cooperation. I look forward to hearing from you with details of how your customerwould like to proceed.

Read More

Abcam Scientific Support

回复于 Jun 28 2012

Question

Product code: 2869
Lot number: GR37744-4

Inquiry: Our customer would like to know what size detected in human sample. Please let me know.

EA hy926 cell

Read More

Abcam community

Verified customer

Asked on Jun 22 2012

Answer

Thank you and your customer for your inquiry.

I can confirm that ab2869 detects a 120 kDa protein representing the sodium / calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. The human protein shows the same molecular weight and I assume that bands at 70 kDa and 160 kDa also present a proteolytic fragment and anon-reduced exchanger respectively.

This publications shows the molecular weight of human NCX1:

Liu J et al. Functional sarcoplasmic reticulum for calcium handling of human embryonic stem cell-derived cardiomyocytes: insights for driven maturation. Stem Cells 25:3038-44 (2007).

This publication also uses milk for blocking and a over night incubation of ab2869.

I therefore recommend to following:

1. ) I suggest to use a positive control. Hek293 cells and human kidney are shown to be NCX1 positive.

2.) I can also suggest to check if the secondary antibody works.

3.) NCX1 is a multi pass protein and I therefore do recommend not to boil the samples but only to heat them up to 70C for 10 minutes.

I hope this information is helpful. please do not hesitate to contactme again with any further questions in this matter.

Read More

Abcam Scientific Support

回复于 Jun 22 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-NCX1 antibody [C2C12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Nov 04 2010

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCX1 antibody [C2C12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal Kidney)
Specification
Normal Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization
Yes - 0.05% Tween20
Blocking step
Normal Goat Serum in 1X PBS (0.05% Tween20) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jul 20 2010

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