Key features and details
- Mouse monoclonal  to NCOR2/SMRT
- Suitable for: ChIP, IHC-Fr, WB, Flow Cyt, IP, IHC-P
- Reacts with: Human
- Isotype: IgG1
参阅全部 NCOR2/SMRT 一抗
描述小鼠单克隆抗体 to NCOR2/SMRT
经测试应用适用于: ChIP, IHC-Fr, WB, Flow Cyt, IP, IHC-Pmore details
- HeLa cell extract
In ChIP ab2780 gives a maximum at 60 min (more than 60%) whereas ab2781 gives a minimum (around 5%) at this time and ab 2780 gives a minimum (less than 5%) at 30 min whereas ab2781 gives a maximum (50%). This protein is corepressor of the nuclear hormone receptors and is not supposed to be recruited in presence of the hormone (here oestrogen), and should be maximal before induction, which is not the case here for both antibodies!
Previously labelled as NCOR2.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
Primary antibody说明Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing Mediator of Retinoic acid & Thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class II / Hda1 Class
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 4 µg/ml. Detects a band of approximately 250, 220 kDa (predicted molecular weight: 274 kDa).Can be blocked with Retinoic Acid Receptor alpha peptide (ab4910).|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
功能Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
组织特异性Ubiquitous. High levels of expression are detected in lung, spleen and brain.
序列相似性Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains.
结构域The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
- Information by UniProt
- CTG 26 antibody
- CTG repeat protein 26 antibody
- CTG26 antibody
Overlay histogram showing HeLa cells stained with ab2781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2781, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
All lanes : Anti-NCOR2/SMRT antibody  (ab2781) at 1/100 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Neuro-2a cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 274 kDa
Observed band size: 220,250 kDa why is the actual band size different from the predicted?
ab2781 被引用在 2 文献中.
- Trümper V et al. Flow cytometry-based FRET identifies binding intensities in PPAR?1 protein-protein interactions in living cells. Theranostics 9:5444-5463 (2019). PubMed: 31534496
- Zhang Z et al. Estrogen-related receptors-stimulated monoamine oxidase B promoter activity is down-regulated by estrogen receptors. Mol Endocrinol 20:1547-61 (2006). PubMed: 16484337