Anti-NCOR2/SMRT抗体[1542] (ab2781)

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Key features and details

  • Mouse monoclonal [1542] to NCOR2/SMRT
  • Suitable for: ChIP, IHC-Fr, WB, Flow Cyt, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-NCOR2/SMRT抗体[1542]
    参阅全部 NCOR2/SMRT 一抗

  • 描述

    小鼠单克隆抗体[1542] to NCOR2/SMRT

  • 宿主

    Mouse

  • 经测试应用

    适用于: ChIP, IHC-Fr, WB, Flow Cyt, IP, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human NCOR2/SMRT aa 994-1005.
    Sequence:

    CGGA-SASDPHREKTQ


    (Peptide available as ab4910)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • HeLa cell extract

  • 常规说明

    In ChIP ab2780 gives a maximum at 60 min (more than 60%) whereas ab2781 gives a minimum (around 5%) at this time and ab 2780 gives a minimum (less than 5%) at 30 min whereas ab2781 gives a maximum (50%). This protein is corepressor of the nuclear hormone receptors and is not supposed to be recruited in presence of the hormone (here oestrogen), and should be maximal before induction, which is not the case here for both antibodies!

    Previously labelled as NCOR2.

性能

应用

Our Abpromise guarantee covers the use of ab2781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 250, 220 kDa (predicted molecular weight: 274 kDa).Can be blocked with Retinoic Acid Receptor alpha peptide (ab4910).
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能

    Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.

  • 组织特异性

    Ubiquitous. High levels of expression are detected in lung, spleen and brain.

  • 序列相似性

    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.

  • 结构域

    The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
    The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CTG 26 antibody
      • CTG repeat protein 26 antibody
      • CTG26 antibody
      • N CoR2 antibody
      • N-CoR2 antibody
      • NCoR 2 antibody
      • Ncor2 antibody
      • NCOR2_HUMAN antibody
      • Nuclear Receptor Co-Repressor 2 antibody
      • Nuclear receptor corepressor 2 antibody
      • retinoic-acid-receptor-associated corepressor antibody
      • Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
      • Silencing mediator of retinoic acid and thyroid hormone receptor antibody
      • SMAP 270 antibody
      • SMAP270 antibody
      • SMRT antibody
      • SMRTE antibody
      • SMRTE tau antibody
      • T3 receptor associating factor antibody
      • T3 receptor-associating factor antibody
      • Thyroid retinoic acid receptor associated corepressor antibody
      • Thyroid- antibody
      • TNRC 14 antibody
      • TNRC14 antibody
      • TRAC 1 antibody
      • TRAC antibody
      • TRAC1 antibody
      see all

    图片

    • Flow Cytometry - Anti-NCOR2/SMRT antibody [1542] (ab2781)
      Flow Cytometry - Anti-NCOR2/SMRT antibody [1542] (ab2781)
      Overlay histogram showing HeLa cells stained with ab2781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2781, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-NCOR2/SMRT antibody [1542] (ab2781)
      Western blot - Anti-NCOR2/SMRT antibody [1542] (ab2781)
      All lanes : Anti-NCOR2/SMRT antibody [1542] (ab2781) at 1/100 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Neuro-2a cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 274 kDa
      Observed band size: 220,250 kDa
      why is the actual band size different from the predicted?

    文献 (2)

    发表研究结果有使用 ab2781？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2781 被引用在 2 文献中.

    • Trümper V  et al. Flow cytometry-based FRET identifies binding intensities in PPAR?1 protein-protein interactions in living cells. Theranostics 9:5444-5463 (2019). PubMed: 31534496
    • Zhang Z  et al. Estrogen-related receptors-stimulated monoamine oxidase B promoter activity is down-regulated by estrogen receptors. Mol Endocrinol 20:1547-61 (2006). PubMed: 16484337

    客户评价及客户问答

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    Question

    I have a question about one of your product. I want to buy an anti-human NCoR antibody, especifically, the NCoR2 antibody (ab2781), and I need to know if this antibody recognize SMRT protein too. I really apreciate if you answer my question quickly,

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    Answer

    Thank you for your enquiry. From my understanding, NCoR2 and SMRT are the same thing. When you look at the SwissProt database entry for NCoR2 (primary accession number Q9Y618), a synonym is SMRT. We do have antibodies to NCoR (thyroid hormone- and retinoic acid receptor-associated corepressor 1) - ab3482 and ab14591. Please let me know if you need additional assistance.

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    Question

    Have these antibodies been tested for cross-reactivity with NcoR1? And what is the difference between these antibodies as the peptides used as the immunogen are the same?

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    Answer

    Both monoclonal products (ab2781 and ab2780) to NCoR2 have the same immunogen: Synthetic Peptide: C G G A(994) S A S D P H R E K T Q(1005). There really is no difference except for the clone. Ab2780 = Cone 1212; Ab2781 = Clone 1542. Unfortunately, we don't have any information regarding the cross-reactivity of these antibodies with I do not have any information as to their cross-reactivity with NcoR1. If you have any additional questions, please contact us again.

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    Question

    I am working with mouse cell's and would like to know if this Ab is good to detect NCoR (ChIP) if not, do you a company thtat produce this Ab. My interest are: NCoR, SMRT and NFkB

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    Answer

    Thank you for your enquiry. Ab2781 has been tested for application in ChIP. However, the antibody has only so far been tested for cross-reactivity with human and has not yet been tested for cross-reactivity with mouse. If you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). If you have any additional questions, please contact us again.

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