Anti-Nck抗体[5B7] (ab129367)
Key features and details
- Mouse monoclonal [5B7] to Nck
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Human, Monkey
- Isotype: IgG1
概述
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产品名称
Anti-Nck抗体[5B7]
参阅全部 Nck 一抗 -
描述
小鼠单克隆抗体[5B7] to Nck -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human, Monkey -
免疫原
Purified recombinant fragment of Human Nck expressed in E. coli(203-371)
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阳性对照
- Jurkat, HeLa, HEK293, A431, K562 and COS7 cell lysates; Jurkat cells. Human rectum cancer tissue; Human Nck (aa203-371) recombinant protein.
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR133551-6 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
5B7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
- COS7 nuclear extract lysate (ab14643)
- HeLa nuclear extract lysate (ab14655)
- Jurkat cytoplasmic extract lysate (ab14842)
- K562 nuclear extract lysate (ab14851)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
- A431 whole cell lysate (ab7909)
- K562 whole cell lysate (ab7911)
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab129367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | 1/500 - 1/2000. Predicted molecular weight: 43 kDa. | |
ELISA | 1/10000. | |
IHC-P | 1/200 - 1/1000. | |
Flow Cyt | 1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. -
序列相似性
Contains 1 SH2 domain.
Contains 3 SH3 domains. -
翻译后修饰
Phosphorylated on Ser and Tyr residues. -
细胞定位
Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments. - Information by UniProt
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数据库链接
- Entrez Gene: 4690 Human
- Omim: 600508 Human
- SwissProt: P16333 Human
- Unigene: 477693 Human
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别名
- Cytoplasmic protein NCK1 antibody
- Melanoma Nck protein antibody
- MGC12668 antibody
see all
图片
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All lanes : Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : Cos7 cell lysate
Predicted band size: 43 kDa -
ab129367 staining Nck antigen by ELISA.
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ab129367, at 1/200 dilution, staining Nck in Jurkat cells by flow cytometry (green). Negative control shown in red.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck antibody [5B7] (ab129367)ab129367, at 1/200 dilution, staining Nck in paraffin-embedded Human rectum cancer tissue by Immunohistochemistry, with DAB staining.
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Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution + Human Recombinant Nck fragment (aa 203-371)
Predicted band size: 43 kDa
The expected molecular weight of the recombinant fragment is 45 kDa.
实验方案
数据表及文件
文献 (0)
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