Anti-Nck抗体[5B7] (ab129367)

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Key features and details

  • Mouse monoclonal [5B7] to Nck
  • Suitable for: WB, ELISA, IHC-P, Flow Cyt
  • Reacts with: Human, Monkey
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Nck抗体[5B7]
    参阅全部 Nck 一抗

  • 描述

    小鼠单克隆抗体[5B7] to Nck

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ELISA, IHC-P, Flow Cytmore details

  • 种属反应性

    与反应: Human, Monkey

  • 免疫原

    Purified recombinant fragment of Human Nck expressed in E. coli(203-371)

  • 阳性对照

    • Jurkat, HeLa, HEK293, A431, K562 and COS7 cell lysates; Jurkat cells. Human rectum cancer tissue; Human Nck (aa203-371) recombinant protein.

  • 常规说明

    This product was changed from ascites to supernatant. Lot no’s high than GR133551-6 are from Tissue Culture Supernatant

性能

应用

Our Abpromise guarantee covers the use of ab129367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.

  • 序列相似性

    Contains 1 SH2 domain.
    Contains 3 SH3 domains.

  • 翻译后修饰

    Phosphorylated on Ser and Tyr residues.

  • 细胞定位

    Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cytoplasmic protein NCK1 antibody
      • Melanoma Nck protein antibody
      • MGC12668 antibody
      • NCK 1 antibody
      • NCK adaptor protein 1 antibody
      • NCK alpha antibody
      • NCK antibody
      • NCK tyrosine kinase antibody
      • Nck-1 antibody
      • NCK1 antibody
      • NCK1_HUMAN antibody
      • NCKalpha antibody
      • Non catalytic region of tyrosine kinase antibody
      • SH2/SH3 adaptor protein NCK alpha antibody
      • SH2/SH3 adaptor protein NCK-alpha antibody
      see all

    图片

    • Western blot - Anti-Nck antibody [5B7] (ab129367)
      Western blot - Anti-Nck antibody [5B7] (ab129367)
      All lanes : Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HEK293 cell lysate
      Lane 4 : A431 cell lysate
      Lane 5 : K562 cell lysate
      Lane 6 : Cos7 cell lysate

      Predicted band size: 43 kDa

    • ELISA - Anti-Nck antibody [5B7] (ab129367)
      ELISA - Anti-Nck antibody [5B7] (ab129367)
      ab129367 staining Nck antigen by ELISA.
    • Flow Cytometry - Anti-Nck antibody [5B7] (ab129367)
      Flow Cytometry - Anti-Nck antibody [5B7] (ab129367)
      ab129367, at 1/200 dilution, staining Nck in Jurkat cells by flow cytometry (green). Negative control shown in red.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck antibody [5B7] (ab129367)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck antibody [5B7] (ab129367)
      ab129367, at 1/200 dilution, staining Nck in paraffin-embedded Human rectum cancer tissue by Immunohistochemistry, with DAB staining.
    • Western blot - Anti-Nck antibody [5B7] (ab129367)
      Western blot - Anti-Nck antibody [5B7] (ab129367)
      Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution + Human Recombinant Nck fragment (aa 203-371)

      Predicted band size: 43 kDa



      The expected molecular weight of the recombinant fragment is 45 kDa.

    文献

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    Question

    concentration d'ab129367

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    Answer

    La concentration d'ab129367 est de 1 mg/ml donc les 100 ul contienent 100 ug d'anticorps.

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