兔单克隆抗体[EPR3333] to Nck-2
Mouse, Rat, Human
Synthetic peptide within Human Nck-2 aa 50-150. The exact sequence is proprietary.
Human spleen lysate, human fetal brain lysate, SH-SY5Y lysate, Human fetal kidney lysate; Human breast ductal carcinoma tissue; HeLa cells
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/100 - 1/250.
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Contains 1 SH2 domain.
Contains 3 SH3 domains.
Cytoplasm. Endoplasmic reticulum.
Information by UniProt
Cytoplasmic protein NCK2 antibody
Growth factor receptor bound protein 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using ab109239 at a dilution of 1/50
Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)
Immunofluorescent staining of HeLa cells using ab109239 at a dilution of 1/100
Western blot - Anti-Nck-2 antibody [EPR3333] (ab109239)
All lanes : Anti-Nck-2 antibody [EPR3333] (ab109239) at 1/1000 dilution Lane 1 : Human spleen lysate Lane 2 : Human fetal brain lysate Lane 3 : SH-SY5Y lysate Lane 4 : Human fetal kidney lysate Lysates/proteins at 10 µg per lane. Predicted band size: 43 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"