Key features and details
- Rabbit polyclonal to NCAM2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 NCAM2 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Rabbit, Horse, Chimpanzee, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human NCAM2 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Human Brain; Mouse Brain; Mouse Olfactory Bulb; Rat Olfactory Bulb.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab130742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa).|
功能May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.
组织特异性Expressed most strongly in adult and fetal brain.
序列相似性Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
- Information by UniProt
- Cell adhesion molecule, neural, 2 antibody
- MGC51008 antibody
- N CAM 2 antibody
All lanes : Anti-NCAM2 antibody (ab130742) at 1 µg/ml
Lanes 1 & 5 : Human brain tissue lysate - total protein (ab29466)
Lanes 2 & 6 : Brain (Mouse) Tissue Lysate
Lanes 3 & 7 : Mouse Olfactory Bulb Lysate
Lanes 4 & 8 : Rat Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
NCAM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.