Anti-NCAM1抗体[ERIC-1] (ab6123)

Submit a review Q&A (1)References (6)

Key features and details

  • Mouse monoclonal [ERIC-1] to NCAM1
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-NCAM1抗体[ERIC-1]
    参阅全部 NCAM1 一抗

  • 描述

    小鼠单克隆抗体[ERIC-1] to NCAM1

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    CD56 positive cells.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab6123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use a concentration of 2 µg/ml.

靶标

  • 功能

    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

  • 序列相似性

    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
      • antigen recognized by monoclonal antibody 5.1H11 antibody
      • CD56 antibody
      • cell adhesion molecule, neural, 1 antibody
      • MSK 39 antibody
      • MSK39 antibody
      • N-CAM-1 antibody
      • NCAM 1 antibody
      • NCAM antibody
      • NCAM C antibody
      • NCAM-1 antibody
      • NCAM1 antibody
      • NCAM1_HUMAN antibody
      • NCAMC antibody
      • Neural cell adhesion molecule 1 antibody
      • Neural cell adhesion molecule NCAM antibody
      • OTTHUMP00000235666 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (ab6123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [ERIC-1] (ab6123)
      Ab6123 staining Human normal skeletal muscle. Staining is localised the membrane.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (ab6123)
      Flow Cytometry - Anti-NCAM1 antibody [ERIC-1] (ab6123)
      Overlay histogram showing HCT116 cells stained with ab6123 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6123, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    文献 (6)

    发表研究结果有使用 ab6123？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6123 被引用在 6 文献中.

    • Mattyasovszky SG  et al. Exposure to radial extracorporeal shock waves modulates viability and gene expression of human skeletal muscle cells: a controlled in vitro study. J Orthop Surg Res 13:75 (2018). PubMed: 29625618
    • Lin JR  et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
    • Althoff K  et al. A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies. Oncogene N/A:N/A (2014). PubMed: 25174395
    • Cunningham C  et al. Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells. PLoS One 8:e83678 (2013). ICC/IF, IHC-Fr ; Rat, Cow . PubMed: 24349544
    • Bourne SP  et al. A monoclonal antibody (ERIC-1), raised against retinoblastoma, that recognizes the neural cell adhesion molecule (NCAM) expressed on brain and tumours arising from the neuroectoderm. J Neurooncol 10:111-9 (1991). PubMed: 1895159
    • Phimister E  et al. Expression of neural cell adhesion molecule (NCAM) isoforms in neuroblastoma. J Clin Pathol 44:580-5 (1991). PubMed: 1856291

    客户评价及客户问答

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    Question

    Phoned to inquire about NCAM antibodies that were specific to human, e.g. did not react to mouse.

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    Answer

    Thank you very much for your interest in ab6132.

    To our knowledge, ab6132 has not been tested in mouse tissues. Therefore, I can offer a discount off a future purchase if you buy ab6123 now, test it inmouse xenograph and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab(xxx) in (UNTESTED APP/SPECIES). I will then send a discount code. This code must be issued before purchasing ab(XXX) so please wait for my reply before ordering.

    2. Purchase either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab6123 turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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