NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen (ab7468)
概述
-
产品名称
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen
参阅全部 Alkaline Phosphatase Chromogen 试剂 -
经测试应用
适用于: WB, Dot blot, IHC-P, IHC-Frmore details -
常规说明
BCIP/TNBT chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in applications such as IHC & immunoblotting. Ready to use formulation
BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride
This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".
性能
-
形式
Liquid -
存放说明
Store at +4°C. -
存储溶液
Constituents: 0.09% NBT, 0.09% 5-bromo-4-chloro-3-indolyl-phosphate-p-toluidine salt -
Concentration information loading...
-
研究领域
相关产品
-
Related Products
- PAP pen (ab2601)
- DAB Enhancer (ab675)
- Normal Donkey Serum (ab7475)
- Normal Chicken Serum (sterile) (ab7477)
- Normal Bovine Calf Serum (sterile) (ab7479)
- Normal Goat Serum (ab7481)
- Normal Guinea Pig Serum (sterile) (ab7482)
- Normal Mouse Serum (sterile) (ab7486)
- Normal Rabbit Serum (Sterile) (ab7487)
- Normal Rat Serum (ab7488)
- Normal Sheep Serum (sterile) (ab7489)
-
Unmasking reagent
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration.
BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light. |
|
Dot blot |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use at an assay dependent concentration. BCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light. |
Dot blot
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab7468 被引用在 3 文献中.
- Gigout A et al. Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species. Arthritis Res Ther 23:113 (2021). PubMed: 33853645
- Hassani A et al. Epstein-Barr virus is present in the brain of most cases of multiple sclerosis and may engage more than just B cells. PLoS One 13:e0192109 (2018). PubMed: 29394264
- Song JW et al. 3'-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli. Sci Rep 6:29406 (2016). WB . PubMed: 27406241