重组Anti-CD93抗体[EPR5386] (ab134079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5386] to CD93
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD93抗体[EPR5386]
参阅全部 CD93 一抗 -
描述
兔单克隆抗体[EPR5386] to CD93 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ELISA,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: U937 (human histiocytic lymphoma monocyte) whole cell lysate, Rat spleen tissue lysate, Mouse spleen tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5386 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134079于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 69 kDa).
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 69 kDa). |
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. -
组织特异性
Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin. -
序列相似性
Contains 1 C-type lectin domain.
Contains 5 EGF-like domains. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 22918 Human
- Entrez Gene: 17064 Mouse
- Entrez Gene: 84398 Rat
- Omim: 120577 Human
- SwissProt: Q9NPY3 Human
- SwissProt: O89103 Mouse
- SwissProt: Q9ET61 Rat
- Unigene: 97199 Human
see all -
别名
- C1q receptor 1 antibody
- C1q/MBL/SPA receptor antibody
- C1qR antibody
see all
图片
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All lanes : Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution
Lane 1 : U937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : Rat spleen tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 100,50-80 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBands around 100kDa and 75kDa are full length CD93 with different level of glycosylation (PMID: 19603257). We are not sure the identity of bands below 70kDa.
The expression profile observed in Raji and 293T is consistent with the literatures (PMID: 31182688, PMID:15459234)
Negative control: Raji and 293T (PMID: 31182688, PMID:15459234)
Blocking and dilution buffer: 5% NFDM/TBST.
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Intracellular flow cytometric analysis of permeabilized THP-1 cells labelling CD93 red with ab134079 at 1/100 and a rabbit IgG negative (green).
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All lanes : Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution
Lane 1 : THP-1 lysate
Lane 2 : Rat kidney lysate
Lane 3 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab134079 被引用在 4 文献中.
- Riether C et al. Metoclopramide treatment blocks CD93-signaling-mediated self-renewal of chronic myeloid leukemia stem cells. Cell Rep 34:108663 (2021). PubMed: 33503440
- Roth M et al. Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells. PLoS One 14:e0210702 (2019). PubMed: 30707726
- Liu W et al. Radioimmunoimaging of 125I-labeled anti-CD93 monoclonal antibodies in a xenograft model of non-small cell lung cancer. Oncol Lett 18:6413-6422 (2019). PubMed: 31819775
- Roth M et al. Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP. PLoS One 12:e0188010 (2017). PubMed: 29182620