The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab94845 is immobilized on an agarose resin via coupling to primary amines to create an affinity resin with specificity for a number of serine proteases.
Provided as 20 ml of 1:1 slurryab94845 binds free trypsin tighter with increasing pH from 4.5-8.0 that allows for a simple gradient to low pH (~2.5-3.0) for elution.
Binding capacity: 1 ml resin binds > 2 mg Trypsin Inhibitor protein
The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.
has not yet been referenced specifically in any publications.