Native小鼠Fibronectin蛋白(ab92784)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
描述
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产品名称
Native小鼠Fibronectin蛋白
参阅全部 Fibronectin 蛋白酶 -
纯度
> 95 % SDS-PAGE.
ab92784 is > 98% pure by SDS-PAGE. Purified from mouse plasma -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Mouse -
预测分子量
220 kDa -
氨基酸
33 to 2477
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab92784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
Thaw the fibronectin by placing the vial in a 37°C water bath and leaving it undisturbed until completely thawed. Do not disturb the vial at any time during the thawing process. If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. Mix very gently with pipette after thawing. Vortexing, excessive agitation, repeated freezing and thawing of fibronectin are not recommended. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 1.58% Tris HCl, 0.87% Sodium chloride
常规信息
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别名
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
功能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
组织特异性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
疾病相关
Glomerulopathy with fibronectin deposits 2 -
序列相似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
发展阶段
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻译后修饰
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab92784 被引用在 6 文献中.
- Zhang J et al. Discovery of a new class of integrin antibodies for fibrosis. Sci Rep 11:2118 (2021). PubMed: 33483531
- Zuo R et al. Rapamycin Induced Autophagy Inhibits Inflammation-Mediated Endplate Degeneration by Enhancing Nrf2/Keap1 Signaling of Cartilage Endplate Stem Cells. Stem Cells 37:828-840 (2019). PubMed: 30840341
- Zhovmer AS et al. The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance. Mol Biol Cell 30:1961-1973 (2019). PubMed: 31318315
- Kashima T et al. Heterogeneous expression patterns of fibronectin in the mouse subiculum. J Chem Neuroanat 98:131-138 (2019). PubMed: 31054323
- Fei D et al. Fibronectin (FN) cooperated with TLR2/TLR4 receptor to promote innate immune responses of macrophages via binding to integrin ß1. Virulence 9:1588-1600 (2018). PubMed: 30272511
- Shen Y et al. Recombinant Decorin Fusion Protein Attenuates Murine Abdominal Aortic Aneurysm Formation and Rupture. Sci Rep 7:15857 (2017). PubMed: 29158532