Native人IgG1蛋白(ab90283)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, ELISA
描述
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产品名称
Native人IgG1蛋白
参阅全部 IgG1 蛋白酶 -
纯度
> 95 % SDS-PAGE. -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Human -
预测分子量
146 kDa -
额外的序列信息
This product is the whole IgG molecule composed of 2 heavy chains and 2 light chains, joined via disulfide bonds.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab90283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
ELISA
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形式
Liquid -
补充说明
Protein Determination: Extinction Coeficient (E) 0.1% at 280nm =1.36.
Prepared from myeloma plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3% Sodium phosphate monobasic, monohydrate
常规信息
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别名
- Ig gamma 1 chain C region
- IgG heavy chain locus
- IGHG1
see all -
相关性
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
细胞定位
Secreted. Cell membrane; Single-pass membrane protein
图片
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The wells were coated with ab200699 at 5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG1 (ab90283) was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab201485 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab90283 被引用在 7 文献中.
- Lu CH et al. The Fc?RIII Engagement Augments PMA-Stimulated Neutrophil Extracellular Traps (NETs) Formation by Granulocytes Partially via Cross-Talk between Syk-ERK-NF-?B and PKC-ROS Signaling Pathways. Biomedicines 9:N/A (2021). PubMed: 34572313
- Deb C et al. A 1-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients. Sci Rep 11:23491 (2021). PubMed: 34873223
- Horns F et al. Memory B Cell Activation, Broad Anti-influenza Antibodies, and Bystander Activation Revealed by Single-Cell Transcriptomics. Cell Rep 30:905-913.e6 (2020). PubMed: 31968262
- Henson D et al. Association Between ApoA-I (Apolipoprotein A-I) Immune Complexes and Adverse Cardiovascular Events-Brief Report. Arterioscler Thromb Vasc Biol 39:1884-1892 (2019). PubMed: 31315438
- Vidal M et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). PubMed: 29397157
- Sinkovits G et al. Concentration and Subclass Distribution of Anti-ADAMTS13 IgG Autoantibodies in Different Stages of Acquired Idiopathic Thrombotic Thrombocytopenic Purpura. Front Immunol 9:1646 (2018). PubMed: 30061898
- MacKay JL & Hammer DA Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin. Integr Biol (Camb) 8:62-72 (2016). PubMed: 26626697