Native人Antithrombin III/ATIII蛋白(ab95077)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: Purification, SDS-PAGE
描述
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产品名称
Native人Antithrombin III/ATIII蛋白
参阅全部 Antithrombin III/ATIII 蛋白酶 -
纯度
> 95 % SDS-PAGE. -
表达系统
Native -
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Human
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技术指标
Our Abpromise guarantee covers the use of ab95077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Purification
SDS-PAGE
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形式
Liquid -
补充说明
2ml of 1:1 slurry
1.0 mg/ml coupled
May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7.4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7.4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage.Previously labelled as Antithrombin III.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1.58% Tris HCl, 0.87% Sodium chloride
常规信息
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别名
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
功能
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
组织特异性
Found in plasma. -
疾病相关
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
序列相似性
Belongs to the serpin family. -
翻译后修饰
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted > extracellular space. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab95077 尚未被引用在任何文献中。