重组Anti-Natriuretic多肽A抗体[EPR22089-283] (ab225844)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22089-283] to Natriuretic peptides A
- Suitable for: IHC-Fr, IHC-P, IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-Natriuretic多肽A抗体[EPR22089-283]
参阅全部 Natriuretic peptides A 一抗 -
描述
兔单克隆抗体[EPR22089-283] to Natriuretic多肽A -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, IHC-P, IP, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse and rat heart tissue lysate. IHC-P: Mouse heart and heart atrium tissues; Rat heart atrium tissue. IHC-Fr: Mouse and rat heart tissues. IP: Mouse heart tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22089-283 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225844于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr |
1/100.
Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa).
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说明 |
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IHC-Fr
1/100. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30. |
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 16 kDa). |
靶标
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功能
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
疾病相关
Atrial standstill 2
Atrial fibrillation, familial, 6 -
序列相似性
Belongs to the natriuretic peptide family. -
翻译后修饰
Cleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 230899 Mouse
- Entrez Gene: 24602 Rat
- SwissProt: P05125 Mouse
- SwissProt: P01161 Rat
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别名
- ANF antibody
- ANF_HUMAN antibody
- ANP antibody
see all
图片
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All lanes : Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 8 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 16291870).
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Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strong cytoplasmic staining mainly in the atria and at lower levels in ventricle of mouse heart (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of mouse atrium is observed.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of rat atrium is observed.
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of mouse atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Natriuretic peptides A was immunoprecipitated from 0.35 mg Mouse heart tissue lysate with ab225844 at 1/1000 dilution. Western blot was perfromed from the immunoprecipitate using ab225844 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Lane 1: Mouse heart lysate 10 μg (Input).
Lane 2: ab225844 IP in Mouse heart tissue lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225844 in Mouse heart lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds -
Immunohistochemical analysis of paraffin-embedded rat heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of rat atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab225844 被引用在 9 文献中.
- Jiang X et al. MCC950 ameliorates ventricular arrhythmia vulnerability induced by heart failure. Bioengineered 13:8593-8604 (2022). PubMed: 35287557
- Gong H et al. Obstructive Sleep Apnea Impacts Cardiac Function in Dilated Cardiomyopathy Patients Through Circulating Exosomes. Front Cardiovasc Med 9:699764 (2022). PubMed: 35321104
- Li Z et al. Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis. Int J Biol Sci 18:2018-2031 (2022). PubMed: 35342336
- Yang Y et al. The lncRNA MIAT regulates CPT-1a mediated cardiac hypertrophy through m6A RNA methylation reading protein Ythdf2. Cell Death Discov 8:167 (2022). PubMed: 35383152
- Liang Q et al. Postnatal Deletion of Bmal1 in Cardiomyocyte Promotes Pressure Overload Induced Cardiac Remodeling in Mice. J Am Heart Assoc 11:e025021 (2022). PubMed: 35730615
- Huo S et al. Alleviation of Inflammation and Oxidative Stress in Pressure Overload-Induced Cardiac Remodeling and Heart Failure via IL-6/STAT3 Inhibition by Raloxifene. Oxid Med Cell Longev 2021:6699054 (2021). PubMed: 33824698
- Xie Y et al. The proteasome activator REG? accelerates cardiac hypertrophy by declining PP2Aca-SOD2 pathway. Cell Death Differ N/A:N/A (2020). PubMed: 32424140
- Cheng Z et al. lncRNA ZEB2-AS1 stimulates cardiac hypertrophy by downregulating PTEN. Exp Ther Med 20:92 (2020). PubMed: 32973941
- Ai F et al. Schisandrin B attenuates pressure overload-induced cardiac remodeling in mice by inhibiting the MAPK signaling pathway. Exp Ther Med 18:4645-4652 (2019). PubMed: 31798701