Native金黄色葡萄球菌蛋白A (HRP) (ab7456)

Submit a review Q&A (2)References (16)

Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy

描述

  • 产品名称

    Native金黄色葡萄球菌蛋白A (HRP)
    参阅全部 Protein A 蛋白酶

  • 纯度

    > 95 % SDS-PAGE.
    Chromatographically pure, a single band by SDS-PAGE.

  • 表达系统

    Native

  • Accession

    P38507

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Native

    • 种属

      Staphylococcus aureus

    • 预测分子量

      56 kDa

  • 偶联物

    HRP

技术指标

Our Abpromise guarantee covers the use of ab7456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    Dot blot

    ELISA

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Frozen sections)

    Immunomicroscopy

  • 形式

    Liquid

  • 补充说明

    Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C.

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1.5% BSA

常规信息

  • 别名

    • gG-binding protein A
    • Immunoglobulin G binding protein A
    • SPA
    • Staphylococcal protein A

  • 相关性

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

  • 细胞定位

    Secreted; cell wall.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (16)

发表研究结果有使用 ab7456？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7456 被引用在 16 文献中.

  • Kuninaga N  et al. Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus). J Vet Med Sci 81:328-337 (2019). PubMed: 30587673
  • Mahajan D  et al. Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking. Nat Commun 10:3218 (2019). PubMed: 31324769
  • Liu J  et al. Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway. J Exp Clin Cancer Res 38:370 (2019). PubMed: 31438997
  • Bhattacharyya T  et al. Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. Curr Biol 29:1002-1018.e7 (2019). PubMed: 30853435
  • Shi M  et al. Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nat Commun 9:4987 (2018). PubMed: 30478271
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-2 of 2 Abreviews or Q&A

Question

Inquiry: Dear Sir/Madam We have purchased abcam Protein A HRP(ab 7456) My query is regarding the dilution of Protein A HRP(ab 7456) to be used in western blotting for development with DAB as substrate
Thanking you Sincerely

Read More
Answer

Thank you for your enquiry.

The best dilution of ab7456 for your application will depend on thethe individual experiment which will require some individual optimization. However, I can suggest dilutions of from 1:1000 and 1:5000 are usually good starting points for optimization .

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Question

band of interest is at about 27 kDda where light chain of endogenous IgG (from tissue samples) appears
send reference for using protein A-HRP or protein G-HRP to not detect the heavy and light chain of endogenous IgG in WB

Read More
Answer

Thank you for contacting us.

Both protein A-HRP (e.g. ab7456) or protein G-HRP (e.g. ab7460) can be used to not detect the heavy and light chain of endogenous IgG in WB.


The links to the datasheets for protein A (HRP) and protein G (HRP) - which would both work well with a rabbit primary antibody are here:

https://www.abcam.com/Protein-A-protein-HRP-ab7456.html
https://www.abcam.com/Protein-G-protein-HRP-ab7460.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com