Native金黄色葡萄球菌蛋白A (HRP) (ab7456)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy
描述
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产品名称
Native金黄色葡萄球菌蛋白A (HRP)
参阅全部 Protein A 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Chromatographically pure, a single band by SDS-PAGE. -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Staphylococcus aureus -
预测分子量
56 kDa
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偶联物
HRP
技术指标
Our Abpromise guarantee covers the use of ab7456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunomicroscopy
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形式
Liquid -
补充说明
Molecular Weight (protein A): 42,000 daltons Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14) -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1.5% BSA
常规信息
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别名
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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相关性
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
细胞定位
Secreted; cell wall.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab7456 被引用在 16 文献中.
- Kuninaga N et al. Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus). J Vet Med Sci 81:328-337 (2019). PubMed: 30587673
- Mahajan D et al. Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking. Nat Commun 10:3218 (2019). PubMed: 31324769
- Liu J et al. Topoisomerase inhibitors promote cancer cell motility via ROS-mediated activation of JAK2-STAT1-CXCL1 pathway. J Exp Clin Cancer Res 38:370 (2019). PubMed: 31438997
- Bhattacharyya T et al. Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes. Curr Biol 29:1002-1018.e7 (2019). PubMed: 30853435
- Shi M et al. Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nat Commun 9:4987 (2018). PubMed: 30478271