Key features and details
- Expression system: Native
- Purity: > 95% Protein A purified
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr
产品名称Native金黄色葡萄球菌蛋白A (Alkaline Phosphatase)
参阅全部 Protein A 蛋白酶
纯度> 95 % Protein A purified.
蛋白长度Full length protein
Our Abpromise guarantee covers the use of ab7345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
补充说明Protein A Concentration: 1.0 mg/ml (by UV absorbance at 280 nm) This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Concentration information loading...
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1.5% BSA
BSA is IgG and Protease free
- gG-binding protein A
- Immunoglobulin G binding protein A
- Staphylococcal protein A
相关性Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
细胞定位Secreted; cell wall.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.