Native猪Trypsin蛋白(ab101090)
Key features and details
- Expression system: Native
- Active: Yes
描述
-
产品名称
Native猪Trypsin蛋白
参阅全部 Trypsin 蛋白酶 -
生物活性
Approximately 5000 Units/mg material.
The spectrophotometric assay to determine the activity of trypsin measures the increase in absorbance at 253 nm due to N-a-benzoyl arginine as it is cleaved from N-a-benzoyl arginine ethyl ester, with the concomitant formation of ethanol. -
表达系统
Native -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
-
种属
Pig -
序列
FPTDDDDKIV GGYTCAANSI PYQVSLNSGS HFCGGSLINS QWVVSAAHCY KSRIQVRLGE HNIDVLEGNE QFINAAKIIT HPNFNGNTLD NDIMLIKLSS PATLNSRVAT VSLPRSCAAA GTECLISGWG NTKSSGSSYP SLLQCLKAPV LSDSSCKSSY PGQITGNMIC VGFLEGGKDS CQGDSGGPVV CNGQLQGIVS WGYGCAQKNK PGVYTKVCNY VNWIQQTIAA N -
预测分子量
24 kDa -
氨基酸
1 to 231
-
技术指标
Our Abpromise guarantee covers the use of ab101090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
形式
Lyophilized -
补充说明
Trypsin catalyzes the cleavage of peptide bonds in proteins at the carboxyl side of arginine and lysine residues, except when either is followed by a proline residue. Trypsin is commonly used to remove adherent cells during cell culture manipulations carried out in vitro.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Store at -20ºC.
Preservative: None
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶This product should ideally be re-desiccated under vacuum over silica gel. Dissolves readily at 1mg/mL in 1 mM Hydrochloric acid, can be used in 67mM potassium phosphate buffer to assay for trypsin activity
常规信息
-
别名
- Alpha-trypsin chain 2
- Anionic trypsinogen
- Beta trypsin
see all -
功能
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
疾病相关
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
翻译后修饰
Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
细胞定位
Secreted > extracellular space. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab101090 尚未被引用在任何文献中。
