Native人IgG FC fragment蛋白(ab90285)

Submit a review Q&A (9)References (6)

描述

  • 产品名称

    Native人IgG FC fragment蛋白
    参阅全部 Human IgG FC fragment 蛋白酶

  • 生物活性

    Shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.

  • 纯度

    > 95 % SDS-PAGE.

  • 表达系统

    Native

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Native

    • 额外的序列信息

      Prepared from plasma

技术指标

Our Abpromise guarantee covers the use of ab90285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 形式

    Liquid

  • 补充说明

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm =1.46.

     

    The IgG fc fragment does not bind antigen; however, it does contain the classic antigenic determinants and biological activity with respect to cytotrophic reactions, binding of complement and catabolic rate control.

     

    Shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 0.05% Sodium azide
    Constituents: 0.79% Tris HCl, 1.16% Sodium chloride

常规信息

  • 相关性

    The Fc region is the constant region on an immunoglobulin molecule, the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens.

图片

  • SDS-PAGE - Native Human IgG FC fragment protein (ab90285)
    SDS-PAGE - Native Human IgG FC fragment protein (ab90285)

    4-12% Bis-Tris NuPAGE Gel analysis of ab90285.

    Lanes 1-3 show 5, 10 and 15 μg, respectively; run under heated/reducing conditions.

    Lanes 4-6 show 5, 10 and 15 μg, respectively; not heated/non-reducing conditions.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献

发表研究结果有使用 ab90285？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab90285 被引用在 6 文献中.

  • de Albuquerque JAT  et al. The Role of AIRE in the Immunity Against Candida Albicans in a Model of Human Macrophages. Front Immunol 9:567 (2018). PubMed: 29666621
  • Kisiswa L  et al. T-type Ca2+ channels are required for enhanced sympathetic axon growth by TNFa reverse signalling. Open Biol 7:N/A (2017). PubMed: 28100666
  • Cohen DJ  et al. Epithelial self-healing is recapitulated by a 3D biomimetic E-cadherin junction. Proc Natl Acad Sci U S A 113:14698-14703 (2016). PubMed: 27930308
  • Kusuda T  et al. Kawasaki disease-specific molecules in the sera are linked to microbe-associated molecular patterns in the biofilms. PLoS One 9:e113054 (2014). PubMed: 25411968
  • DeLelys ME  et al. Relationship between ABO genotype and A antigen expression on platelets. Transfusion 53:1763-71 (2013). PubMed: 23145947
  • Jonigk D  et al. Anti-inflammatory and immunomodulatory properties of a1-antitrypsin without inhibition of elastase. Proc Natl Acad Sci U S A 110:15007-12 (2013). PubMed: 23975926

客户评价及客户问答

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1-9 of 9 Abreviews or Q&A

Question

My question was whether the Fc fragments were received from a pool of polyclonal or monoclonal antibodies.

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Answer


We prepare IgG, FC Fragment from polyclonal IgG.

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Question

The FC fragment of IgG contains a glycosylation site. Do you de-glycosylate (remove the carboxyl group) or know what the glycyosylation status is?

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Answer

We do not know the glycosylation status of the IgG FC fragment as we do not test for this as part of our final QC analysis. We do not actively attempt to deglycosylate the FC as part of the purification procedure. I am including a general description of the purification protocol to support that we do not believe that the procedure actively deglycosylated the FC. The whole molecule IgG is purified by ART using ammonium sulfate precipitation, and ion exchange chromatography. Protein A chromatography is not used for the purification of IgG.

IgG Fc purification

1. Digestion of whole molecule IgG with papain.

2. Ion exchange chromatography

3. Size exclusion chromatography

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Question

I have been working with one of your proteins (Human IgG Fc fragment protein; Ref. ab90285) for a while now and was wondering if you could provide me with more detailed information regarding the structure of your protein please? I did refer to the product datasheet but I would be very grateful for more details if possible.

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Answer

Unfortunately, we do not have information on the structure of the protein.

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Question

This customer raised an enquire about ab90285 (Human IgG FC fragment protein (Human)), she would like to ask for your help to know the approximate concentration range of this antibody, could you please help this customer to solve the problem?

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Answer

For the recent batch we sell, ab90285 lot# GR90653 is 2.1 mg/ml.

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Question

This is what we have been using instead:
https://www.abcam.com/Human-IgG-FC-fragment-protein-Human-ab90285.html
Would the new product you could provide be a more appropriate control or less expensive?

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Answer

Thank you for your reply.

I am confident that the reference ab90285 is a suitable negative control for ab108557.
Furthermore, I have just been informed about the price of the new Fc fragments which is $424.00 for 100µg, therefore I would recommend to keep using the much cheaper Fc fragments reference ab90285, $269.00 for 1mg.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

endotoxin levels determined?
can use for cell culture as control?

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Answer

Thank you for contacting us.

The lab let me know that they have not measured the endotoxin levels for this product.
Also, they do not know whether or not it could be used a s a control in cell culture.

However, if you would like to try it out, we have a testing discount program that you could use in that regard. Here is some more information:

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This program applies to ELISA kits and other kits we offer.


Please let me know if you would like to receive a testing discount code for trying the protein in cell culture.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I’m wondering how sensitive the immunoelectrophoresis is to see residual contamination of Fab if there is any, can you detect 1% contamination from this technique?

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Answer

Thank you for your reply.

While we don't have an exact figure for how sensitive the immunoelectrophoresis is, we do not believe the protein will contain 1% contamination with F(ab). I hope this helps, please let me know if you need any other information.

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Question

Has this IgG Fc fragment protein been checked for contamination with F(ab) fragments?
Do you have any other Fc fragment proteins with higher purity than 95%?

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Answer

Thank you for contactingus.

This Fc fragment protein shows no reaction to F(ab) on immunoelectrophoresis. We will guarantee this product to be free from F(ab) fragment contamination. Unfortunately, we do not currently have any other purified human IgG Fc fragment proteins in our catalog.

I hope this helps, please let me know ifyou need any additional information.

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Question

Is this a fragment of an IgG1 antibody subclass?

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Answer

We prepare ab90285 from human plasma having a mixture of IgG1, IgG2, IgG3 and IgG4.  After digestion and purification, the final product should similarly be a mixture of the Fc regions for the four subclasses.

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