Native Carcino Embryonic Antigen CEA蛋白(ab742)

Submit a review Q&A (7)References (5)

Key features and details

  • Expression system: Native
  • Suitable for: ELISA

描述

技术指标

Our Abpromise guarantee covers the use of ab742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

  • 形式

    Liquid

  • 补充说明

    Blood samples from tissue donors were tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV.SDS-PAGE denaturating : multiple protein bands 150 – 210 kDa

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: 2% Methyl mannoside

    0.1 M PBS

常规信息

  • 别名

    • Carcinoembryonic antigen
    • Carcinoembryonic antigen-related cell adhesion molecule 5
    • CD66e
    • CEA
    • Ceacam5
    • CEAM5_HUMAN
    • DKFZp781M2392
    • Meconium antigen 100
    • OTTHUMP00000199032
    • OTTHUMP00000199033
    • OTTHUMP00000199034
    see all

  • 功能

    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.

  • 组织特异性

    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

  • 序列相似性

    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.

  • 翻译后修饰

    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (5)

发表研究结果有使用 ab742？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab742 被引用在 5 文献中.

  • Yen YK  et al. A Graphene-PEDOT:PSS Modified Paper-Based Aptasensor for Electrochemical Impedance Spectroscopy Detection of Tumor Marker. Sensors (Basel) 20:N/A (2020). PubMed: 32131502
  • Liu J  et al. Integrating Metal-Enhanced Fluorescence and Surface Acoustic Waves for Sensitive and Rapid Quantification of Cancer Biomarkers from Real Matrices. ACS Sens 3:222-229 (2018). PubMed: 29284267
  • Sato M  et al. Development and validation of scFv-conjugated affinity silk protein for specific detection of carcinoembryonic antigen. Sci Rep 7:16077 (2017). PubMed: 29167497
  • Oberst MD  et al. CEA/CD3 bispecific antibody MEDI-565/AMG 211 activation of T cells and subsequent killing of human tumors is independent of mutations commonly found in colorectal adenocarcinomas. MAbs 6:1571-84 (2014). PubMed: 25484061
  • Li Y  et al. Carcinoembryonic antigen interacts with TGF-{beta} receptor and inhibits TGF-{beta} signaling in colorectal cancers. Cancer Res 70:8159-68 (2010). PubMed: 20889724

客户评价及客户问答

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1-7 of 7 Abreviews or Q&A

Question

Hello,

I have recently purchased one of your products: carcinoembryonic antigen protein CEA (ab742).

I would like to know what is the source of this product (i.e. recombinant/human cell line/primary tissue/other) and whether or not it contains post-translational modifications such as glycosylation.

Thanks,

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Answer

Thank you for contacting us and your interest in our products.

The Carcino Embryonic Antigen CEA protein (ab742) is isolated from colon carcinoma liver metastatic tissue. The protein is therefore in its native form and should maintain the post-translational modifications such as glycosylation.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

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Question

Dear technical team,

Our customer would like to purchase ab4451 and ab15987 for sELISA and will apply ab742 as a standard.
I checked ab742 doesn’t guarantee for ELISA but let me know if it is possible.

Best regards,

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Answer

Thank you for your enquiry.

I can confirm that ab4451 and ab15987 have been tested and guarantee for use in sELISA together according to the datasheets. Theab742 Carcino Embryonic Antigen CEA protein is full length native protein so should be suitable as a standard for use with this antibodies.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

our user ordered ab742. could you please let us know isoelectric point of this? if you do not test with this, please let us know sequence of this proteins. our user would like to calculate it in person. additionally, could you please let us know how pure this protien is? please check this for our user. thank you so much for your consideration all the time. I look forward to hearing from you.

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Answer

Thank you for your enquiry. Unfortunately, we have not determined the isoelectric point or the sequence for this product. The product is purified from colon carcinoma liver metastatic tissue and the purity is 95% by SDS-PAGE. I hope this information will still be useful. If there is anything else that I can help you with, please do not hesitate to contact me.

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Question

Can you tell me if these products can be used in a sandwich ELISA? ＃AB10039　Carcino Embryonic CEA antibody　 ＃AB10039 Carcino Embryonic CEA antibody ＃AB742 Carcino Embryonic Antigen protein　

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Answer

Thank you for your enquiry. Products ab10036 and ab10039 work as a pair in sandwich immunoassay, but we have not tested them with ab742 antigen, so I am not able to comment about that. If you decide to go ahead and purchase the products, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

I don't understand what "Epitope specificity group IV, IVa, or VI" mean? What is NCA2? I have one more question is that if these antibodies only recognize glycosylated CEA protein? Because I will express CEA in yeast as unglyxosylated protein, will it be recognized by any of your antibodies? I already ordered ab742 CEA antigen and ab10039 CEA antibody, and the WB showed the CEA protein are smear bands from 75-210 kDa. I want to know what the 5kDa part is? degradation or in completed glycosylated CEA? I attached the picture of the WB.

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Answer

Thank you for your enquiry and patience. We unfortunately do not have any additional information with regards to the epitope specificity(which was given per ISOBM classification). NCA2 is nonspecific cross-reacting antigen 2. Ab742 is suitable as immunogen for antisera production, tracer for iodination and standard for immunoassay. We don't have any information regarding its use with ab10039 in Western blotting. If you have any additional questions, please contact us again.

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Question

I have questions about the CEA antibodies and CEA proteins. You have 4 CEA mouse antidodies which could be used in WB. They are AB10038,10039, 10037, and 11330. I want to know what part of the CEA that these antidoies recogonize. i will express full length and truncated CEA in yeast and want to detect the expression by WB. The truncated CEA doesn't have the 34aa N terminal signal peptide and 16aa c terminal hydrophobic domain, and there for won't be glycosylated; which of your antidoies will recognize it? I also want to buy a purified CEA protein as a positive control in WB, you also have two in your product list, which are ab742 and ab2282; i didn't find the differences between them, and which one you think could be used as a positive standard in WB. and what it will like in WB, signal band or a smear band?

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Answer

Thank you for your enquiry. For the following antibodies, epitope specificity is given per ISOBM classification and we have only following information: Ab10038 - Epitope specificity group IV, cross-reactive with NCA2. Ab10039 - Epitope specificity group IV. Ab10037 - Epitope specificity group IVa or IV/VI. Ab11330 - The epitope has not been determined; this antibody reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissues including colorectal, lung and breast tumors. Ab10038 is not suitable for use in Western blotting. Ab742 is suitable as immunogen for antisera production, tracer for iodination and standard for immunoassay. Specificity was determined by SDS-PAGE denaturating: multiple protein bands 150 - 210 kDa. Ab2282 is the mitogen free version of ab742. Ab2282 is suitable as an immunogen for antisera production, a tracer for iodination and as a standard for immunoassays. It is specifically depleted from traces of mitogens, therefore it is suitable for in vitro and in vivo studies of specific immune responses (vaccine trials).

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Question

Is Ab 742 the full-length, i.e. with intact GPI membrane anchor, protein CEA, or some secreted or cleaved form lacking the membrane anchor?

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Answer

The CEA antigen is purified from a single patient source, colon carcinoma liver metastatic tissue, by immunoaffinity purification. Unfortunately we have not checked the presence of membrane anchor in the preparation.

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