Anti-NAIP抗体(ab2549)
Key features and details
- Rabbit polyclonal to NAIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NAIP抗体
参阅全部 NAIP 一抗 -
描述
兔多克隆抗体to NAIP -
宿主
Rabbit -
特异性
This antibody detects recombinant human NAIP in extracts from Sf21 insect cells, but it is unknown as to whether this antibody detects the endogenous form. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide surrounding amino acid 506 of human NAIP.
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常规说明
NAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
NAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa. |
靶标
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功能
Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy. -
组织特异性
Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord. -
序列相似性
Contains 3 BIR repeats.
Contains 1 NACHT domain. - Information by UniProt
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数据库链接
- Entrez Gene: 100510692 Human
- Entrez Gene: 4671 Human
- Omim: 600355 Human
- SwissProt: Q13075 Human
- Unigene: 654500 Human
- Unigene: 710305 Human
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别名
- Baculoviral IAP repeat containing 1 antibody
- Baculoviral IAP repeat-containing protein 1 antibody
- BIRC 1 antibody
see all
图片
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Western blot - Anti-NAIP antibody (ab2549)Image from Dr MT Romanish et al, PLoS One. 2009 Jun 2;4(6):e5761, Fig 5.All lanes : Anti-NAIP antibody (ab2549) at 1/1000 dilution
Lane 1 : Lysate prepared from transfected PC3 cells.
Lane 2 : Lysate prepared from non-transfected PC3 cells.
Lane 3 : Lysate prepared from transfected HeLa cells.
Lane 4 : Lysate prepared from non-transfected HeLa cells.
Lane 5 : Lysate prepared from non-transfected NTera2D1 cells.
Secondary
All lanes : secondary antibody at 1/100000 dilution
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible isoform), 115 kDa, 130 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Detection of novel NAIP protein isoforms.Cells were grown in 10 cm dishes. The human PC3, NTera2D1, and HeLa cell lines were selected to screen for NAIP proteins based on preliminary RT-PCR findings. Cells transfected with the expression vector encoding the Alu-derived NAIP ORF or untransfected controls were harvested by either scraping or trypsinization following two washes with cold PBS. Cell pellets were obtained by centrifugation and resuspended in RIPA (150 mM NaCl; 1% NP-40; 0.5% sodium deoxycholate; 0.1% SDS; 50 mM Tris, pH8) and NP40 (150 mM NaCl; 1% NP-40; 50 mM Tris, pH8) lysis buffers supplemented with a protease inhibitor cocktail, and subsequently quantified using the Qubit Fluorometer. RIPA provided clearer results for the NAIP-specific antibody. Bi-phased gels containing TEMED and APS (4% stacking, 9% separating) were used to resolve total cellular protein in electrophoresis running buffer (10×: 25 mM Tris; 192 mM glycine; 0.1% SDS). Subsequently, separa
数据表及文件
文献 (1)
ab2549 被引用在 1 文献中.
- Romanish MT et al. A novel protein isoform of the multicopy human NAIP gene derives from intragenic Alu SINE promoters. PLoS One 4:e5761 (2009). WB ; Human . PubMed: 19488400
