Application
Western blot
Sample
Mouse Tissue lysate - other (Mouse cortex)
Loading amount
15 µg
Specification
Mouse cortex
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Concentration
3 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk PBS 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit IgG HRP conjugate Upstate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Immobilon western blot detection reagent
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 160 kDa Non-specific: 50, 60 kDa
Additional data
Additional Notes
Lane 1 - nNOS pS847 ab16650 batch 363718 1ug/mL
Lane 2 - nNOS pS847 ab16650 batch 363718 3ug/mL
Lane 3 - nNOS pS847 ab16650 batch 363718 3ug/mL+ phosphopeptide ab16981 3ug/mL
Lane 4 - nNOS pS847 ab16650 batch 363718 3ug/mL+ unmodified peptide ab16986 3ug/mL
Lane 5 - nNOS pS847 ab16650 batch 363718 10ug/mL
Lane 2 - nNOS pS847 ab16650 batch 363718 3ug/mL
Lane 3 - nNOS pS847 ab16650 batch 363718 3ug/mL+ phosphopeptide ab16981 3ug/mL
Lane 4 - nNOS pS847 ab16650 batch 363718 3ug/mL+ unmodified peptide ab16986 3ug/mL
Lane 5 - nNOS pS847 ab16650 batch 363718 10ug/mL
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 05 2009