Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Frozen Brain sections)
Specification
Frozen Brain sections
Fixative
Formalin
Permeabilization
Yes - Triton X-100 (0.25%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + TritonX-100 + normal donkey serum
Secondary antibody
Name
Non-Abcam antibody was used: biotin-conjugated IgG donkey anti-rabbit (Jackson
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
PubMed ID
20881244: View
Additional Notes
Excellent antibody for immunohistochemistry (peroxidase reaction using DAB) in mouse frozen brain sections. Very low background.
Results using this antibody is published in:
Donato J Jr, Frazão R, Fukuda M, Vianna C, Elias CF. Leptin induces phosphorylation of neuronal nitric oxide synthase in defined hypothalamic neurons. Endocrinology November 2010, 151(11):0000–0000.
Results using this antibody is published in:
Donato J Jr, Frazão R, Fukuda M, Vianna C, Elias CF. Leptin induces phosphorylation of neuronal nitric oxide synthase in defined hypothalamic neurons. Endocrinology November 2010, 151(11):0000–0000.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Jose Donato
Verified customer
提交于 Oct 22 2010