Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0,5% Milk 5% NaN3
Dilution
1/200
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-goat HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Clarity
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 15, 130 kDa
Additional data
Additional Notes
Lane 1: ladder
Lane 2: mouse intestinal tissue lysate
Lane 3: rat intestinal tissue lysate
Lane 4: Human intestinal tissue lysate
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
In human, the Isoform 3 (identifier: P29475-3), also known as: TN-NOS or TN-NOSB, would migrate at 125 kDa. The observed band at 130 kDa may correspond to this isoform.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 18 2016