Anti-Myosin VIIa/MYO7A抗体(ab3481)
Key features and details
- Rabbit polyclonal to Myosin VIIa/MYO7A
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Guinea pig, Cow, Human
- Isotype: IgG
概述
-
产品名称
Anti-Myosin VIIa/MYO7A抗体
参阅全部 Myosin VIIa/MYO7A 一抗 -
描述
兔多克隆抗体to Myosin VIIa/MYO7A -
宿主
Rabbit -
特异性
Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A overexpressed in Sf9 insect cell lysate.
-
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Guinea pig, Cow, Human -
免疫原
Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
Sequence:SGQEFDVPIGAVVKLC
(Peptide available asab4996) -
常规说明
This product was previously labelled as Myosin VIIa
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 3-5µg for 106 cells. | |
| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa. |
靶标
-
相关性
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle. -
细胞定位
Cytoplasmic, cytoskeleton -
数据库链接
- Entrez Gene: 4647 Human
- Entrez Gene: 17921 Mouse
- Omim: 276903 Human
- SwissProt: Q13402 Human
- SwissProt: P97479 Mouse
- Unigene: 370421 Human
- Unigene: 1403 Mouse
-
别名
- Deafness autosomal dominant 11 antibody
- Deafness autosomal recessive 2 antibody
- DFNA11 antibody
see all
图片
-
Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution -
Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.
-
Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control
实验方案
文献 (19)
ab3481 被引用在 19 文献中.
- Mei H et al. Increased mitochondrial DNA copy number protects hair cells and HEI-OC1 cells against drug-induced apoptosis. Mol Med Rep 21:338-346 (2020). PubMed: 31939628
- Lee SY et al. Outcomes of Peptide Vaccine GV1001 Treatment in a Murine Model of Acute Noise-Induced Hearing Loss. Antioxidants (Basel) 9:N/A (2020). PubMed: 32012778
- Li S et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
- Wen Q et al. Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis. Endocrinology 160:484-503 (2019). PubMed: 30649248
- Xu W et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420
