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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin

Anti-Myosin VIIa/MYO7A抗体(ab3481)

  • Datasheet
  • SDS
Reviews (9)Q&A (3)References (19)

Product price, shipping and contact information

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Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
  • Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
  • Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)

Key features and details

  • Rabbit polyclonal to Myosin VIIa/MYO7A
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Guinea pig, Cow, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Myosin VIIa/MYO7A抗体
    参阅全部 Myosin VIIa/MYO7A 一抗
  • 描述

    兔多克隆抗体to Myosin VIIa/MYO7A
  • 宿主

    Rabbit
  • 特异性

    Detects Myosin VIIa/MYO7A from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa/MYO7A from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa/MYO7A  overexpressed in Sf9 insect cell lysate.

  • 经测试应用

    适用于: Flow Cyt, WBmore details
  • 种属反应性

    与反应: Mouse, Guinea pig, Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Myosin VIIa/MYO7A aa 16-31.
    Sequence:

    SGQEFDVPIGAVVKLC


    (Peptide available as ab4996)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

     This product was previously labelled as Myosin VIIa

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Myosin VIIa/MYO7A protein (ab152555)

应用

Our Abpromise guarantee covers the use of ab3481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 3-5µg for 106 cells.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.

靶标

  • 相关性

    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
  • 细胞定位

    Cytoplasmic, cytoskeleton
  • 数据库链接

    • Entrez Gene: 4647 Human
    • Entrez Gene: 17921 Mouse
    • Omim: 276903 Human
    • SwissProt: Q13402 Human
    • SwissProt: P97479 Mouse
    • Unigene: 370421 Human
    • Unigene: 1403 Mouse
    • 别名

      • Deafness autosomal dominant 11 antibody
      • Deafness autosomal recessive 2 antibody
      • DFNA11 antibody
      • DFNB 2 antibody
      • DFNB2 antibody
      • Myo7a antibody
      • Myosin 7a antibody
      • Myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)) antibody
      • Myosin VIIa antibody
      • Myosin, unconventional, family VII, member A antibody
      • MYOVIIA antibody
      • MYU7A antibody
      • NSRD 2 antibody
      • NSRD2 antibody
      • Unconventional myosin VIIa antibody
      • Ush 1B antibody
      • Ush1b antibody
      • Usher syndrome 1B antibody
      see all

    图片

    • Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Anti-Myosin VIIa/MYO7A antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %

      Secondary
      HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution
    • Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Western blot - Anti-Myosin VIIa/MYO7A antibody (ab3481)

      Western blot detection of Myosin VIIa/MYO7A in mouse testes tissue extract using ab3481.

    • Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)
      Flow Cytometry - Anti-Myosin VIIa/MYO7A antibody (ab3481)

      ab3481 staining Myosin VIIa/MYO7A (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control

       

    实验方案

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (19)

    发表研究结果有使用 ab3481?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3481 被引用在 19 文献中.

    • Mei H  et al. Increased mitochondrial DNA copy number protects hair cells and HEI-OC1 cells against drug-induced apoptosis. Mol Med Rep 21:338-346 (2020). PubMed: 31939628
    • Lee SY  et al. Outcomes of Peptide Vaccine GV1001 Treatment in a Murine Model of Acute Noise-Induced Hearing Loss. Antioxidants (Basel) 9:N/A (2020). PubMed: 32012778
    • Li S  et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
    • Wen Q  et al. Myosin VIIa Supports Spermatid/Organelle Transport and Cell Adhesion During Spermatogenesis in the Rat Testis. Endocrinology 160:484-503 (2019). PubMed: 30649248
    • Xu W  et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 12 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Myosin VIIa antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Cochlea)
    Specification
    Cochlea
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 10 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin VIIa antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Whole Mouse Foetus Section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    Yes - Triton X-100
    Specification
    Whole Mouse Foetus Section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 16 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (MDCK II)
    Specification
    MDCK II
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    提交于 Nov 27 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Retinal Pigment Epithelium primary cells)
    Specification
    Retinal Pigment Epithelium primary cells
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    提交于 Nov 26 2009

    Question

    Expiry data and availability

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2009

    Answer

    Thank you for your enquiry. There is no stated expiration date for this antibody. There is one year expiration date from the date of purchase, however the Abpromise will be still effective for only 120 days after purchase. This antibody is unlikely to be be discontinued as the sales for the last two years have been consistent. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Nov 18 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary cell culture, retinal pigment epithelium)
    Specification
    Primary cell culture, retinal pigment epithelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    提交于 Dec 02 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Hamster Cell (CHO, chinese hamster ovary cells)
    Specification
    CHO, chinese hamster ovary cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    提交于 Dec 02 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (ear cross sections)
    Specification
    ear cross sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Read More

    Mrs. Chantal Longo-Guess

    Verified customer

    提交于 Jun 10 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (Primary cell culture, retinal pigment epithelium)
    Specification
    Primary cell culture, retinal pigment epithelium
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TX100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    提交于 Jun 05 2008

    Western blot abreview for Anti-Myosin VIIa antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (Pig RPE cells)
    Loading amount
    25 µg
    Specification
    Pig RPE cells
    Gel Running Conditions
    Reduced Denaturing (7%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    提交于 Jun 03 2008

    1-10 of 12 Abreviews or Q&A

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