Anti-Myosin light chain (phospho S20)抗体(ab2480)
Key features and details
- Rabbit polyclonal to Myosin light chain (phospho S20)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Myosin light chain (phospho S20)抗体
参阅全部 Myosin light chain 一抗 -
描述
兔多克隆抗体to Myosin light chain (phospho S20) -
宿主
Rabbit -
特异性
This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Customers have seen variable performance with Xenopus laevis.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Human Myosin Light Chain phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - KKRPQRAT(pS)NVFAMFD (aa 12-27).
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab2480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 20 kDa. We recommend using BSA for blocking when using phospho-specific antibodies. |
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| IHC-P | Use a concentration of 2.5 µg/ml. |
靶标
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功能
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
组织特异性
Smooth muscle tissues and in some, but not all, nonmuscle cells. -
序列相似性
Contains 3 EF-hand domains. -
翻译后修饰
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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数据库链接
- Entrez Gene: 10398 Human
- Entrez Gene: 98932 Mouse
- Omim: 609905 Human
- SwissProt: P24844 Human
- SwissProt: Q9CQ19 Mouse
- Unigene: 504687 Human
- Unigene: 271770 Mouse
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别名
- 20 kDa myosin light chain antibody
- KMLC antibody
- LC20 antibody
see all
图片
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Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)All lanes : Anti-Myosin light chain (phospho S20) antibody (ab2480) at 1/1000 dilution
Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
Lane 2 : Regulatory Light Chain Phospho recombinant protein, 50 ng
Lane 3 : Smooth Muscle Non-Phospho recombinant protein, 50 ng
Lane 4 : Smooth Muscle Phospho recombinant protein, 50 ng
Secondary
All lanes : Peroxidase rabbit secondary antibody at 1/40000 dilution
Predicted band size: 20 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain (phospho S20) antibody (ab2480)IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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Western blot - Anti-Myosin light chain (phospho S20) antibody (ab2480)Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA treated:
Lane 1 : untreated 45 min
Lane 2 : CLA 50 nm 45 min
Lane 3 : CLA 100 nm 45 min
Lane 4 : A21 untreated 45 min
Lane 5 : A22 A23187 5 min
Lane 6 : A23 A23187 15 min
Lane 7 : A24 A23187 60 min
After washing, a 1/5,000 dilution of anti-rabbit HRP (ab7090) was used as secondary.Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20
实验方案
数据表及文件
文献 (96)
ab2480 被引用在 96 文献中.
- Ding J et al. Myosin light chain kinase inhibitor ML7 improves vascular endothelial dysfunction and permeability via the mitogen-activated protein kinase pathway in a rabbit model of atherosclerosis. Biomed Pharmacother 128:110258 (2020). PubMed: 32516749
- Guo C et al. Bilobalide reversibly modulates blood-brain barrier permeability through promoting adenosine A1 receptor-mediated phosphorylation of actin-binding proteins. Biochem Biophys Res Commun 526:1077-1084 (2020). PubMed: 32312522
- Meyer K et al. Bile canaliculi remodeling activates YAP via the actin cytoskeleton during liver regeneration. Mol Syst Biol 16:e8985 (2020). PubMed: 32090478
- Chen AS et al. Drak/STK17A Drives Neoplastic Glial Proliferation through Modulation of MRLC Signaling. Cancer Res 79:1085-1097 (2019). PubMed: 30530503
- Marivin A et al. GPCR-independent activation of G proteins promotes apical cell constriction in vivo. J Cell Biol 218:1743-1763 (2019). PubMed: 30948426
