Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Muscles)
Specification
Muscles
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX100
Other product details
Concentration
1 (unknown units)
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% PBS plus TritonX100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 568
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Mouse
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
ICC/IF image of adult mouse muscle section stained with ab1835. The animals were perfused with 4% PFA. The sections were incubated in 5% normal donkey serum in 0.1% PBS- and triton X100 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab1835, 1µg/ml) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse IgG (H+L) (red) was used at a 1/1000 dilution for 1h.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 May 19 2010