重组Anti-MYL9抗体[EPR13013(2)(B)] (ab187152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13013(2)(B)] to MYL9
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-MYL9抗体[EPR13013(2)(B)]
参阅全部 MYL9 一抗 -
描述
兔单克隆抗体[EPR13013(2)(B)] to MYL9 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and human colon cell lysate; Human stomach, colon and uterus lysates; Mouse muscle lysate. IHC-P: Human colon tissue; Mouse stomach tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13013(2)(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab187152于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
组织特异性
Smooth muscle tissues and in some, but not all, nonmuscle cells. -
序列相似性
Contains 3 EF-hand domains. -
翻译后修饰
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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数据库链接
- Entrez Gene: 10398 Human
- Entrez Gene: 98932 Mouse
- Entrez Gene: 296313 Rat
- Omim: 609905 Human
- SwissProt: P24844 Human
- SwissProt: Q9CQ19 Mouse
- SwissProt: Q64122 Rat
- Unigene: 504687 Human
see all -
别名
- 20 kDa myosin light chain antibody
- Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
- LC20 antibody
see all
图片
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All lanes : Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MYL9 knockout HeLa cell lysate
Lane 3 : Human colon cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDaLanes 1-4: Merged signal (red and green). Green - ab187152 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab187152 Anti-MYL9 antibody [EPR13013(2)(B)] was shown to specifically react with MYL9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266036 (knockout cell lysate ab256999) was used. Wild-type and MYL9 knockout samples were subjected to SDS-PAGE. ab187152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MYL9 with ab187152 at 1/500 dilution. Anti-rabbit IgG HRP was used as a secondary antibody and slides were counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MYL9 with ab187152 at 1/500 dilution. Anti-rabbit IgG HRP was used as a secondary antibody and slides were counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/1000 dilution + Mouse muscle lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa -
All lanes : Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/5000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Human colon lysate
Lane 3 : Human uterus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 20 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab187152 被引用在 1 文献中.
- Fei L et al. Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development. Nat Genet 54:1051-1061 (2022). PubMed: 35817981