重组Anti-Myeloperoxidase抗体[EPR4793] (ab134132)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4793] to Myeloperoxidase
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Myeloperoxidase抗体[EPR4793]
参阅全部 Myeloperoxidase 一抗 -
描述
兔单克隆抗体[EPR4793] to Myeloperoxidase -
宿主
Rabbit -
特异性
The antibody recognizes Myeloperoxidase (85-90 kDa) and its heavy chain (60 kDa). -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4793 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134132于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at dilution between 1/250 to 1/500 |
说明 |
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Flow Cyt (Intra)
1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at dilution between 1/250 to 1/500 |
靶标
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功能
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
疾病相关
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
序列相似性
Belongs to the peroxidase family. XPO subfamily. -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 4353 Human
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
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别名
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
图片
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Anti-Myeloperoxidase antibody [EPR4793] (ab134132) at 1/1000 dilution (Purified) + HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 83 kDa -
Anti-Myeloperoxidase antibody [EPR4793] (ab134132) at 1/1000 dilution (Purified) + Human spleen lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 83 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Myeloperoxidase with Purified ab134132 at 1:1000 dilution (0.2 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HL-60 (Human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with purified ab134132 at 1/10000 dilution (0.1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Negative control: HeLa.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab134132 被引用在 8 文献中.
- Zhang A et al. Lactate-induced M2 polarization of tumor-associated macrophages promotes the invasion of pituitary adenoma by secreting CCL17. Theranostics 11:3839-3852 (2021). PubMed: 33664865
- Kuang Y et al. New prognostic factors and scoring system for patients with acute myeloid leukemia. Oncol Lett 22:823 (2021). PubMed: 34691250
- Zhang H et al. Preoperative leukocytosis is associated with increased tumor-infiltrating neutrophil extracellular traps and worse outcomes in esophageal cancer. Ann Transl Med 8:441 (2020). PubMed: 32395485
- Wan J et al. Inhibition of miR-155 reduces impaired autophagy and improves prognosis in an experimental pancreatitis mouse model. Cell Death Dis 10:303 (2019). PubMed: 30944299
- Her Z et al. An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia. J Hematol Oncol 10:162 (2017). IHC-P ; Mouse . PubMed: 28985760
- Atwal M et al. Myeloperoxidase Enhances Etoposide and Mitoxantrone-Mediated DNA Damage: A Target for Myeloprotection in Cancer Chemotherapy. Mol Pharmacol 91:49-57 (2017). WB . PubMed: 27974636
- Nayak RC et al. Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells. J Clin Invest 125:3103-16 (2015). IF . PubMed: 26193632
- Han S et al. Pre-treatment neutrophil-to-lymphocyte ratio is associated with neutrophil and T-cell infiltration and predicts clinical outcome in patients with glioblastoma. BMC Cancer 15:617 (2015). IHC . PubMed: 26341881