重组Anti-Myelin PLP抗体[plpc 1] (ab9311)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [plpc 1] to Myelin PLP
- Suitable for: WB, IHC-Fr, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-Myelin PLP抗体[plpc 1]
参阅全部 Myelin PLP 一抗 -
描述
小鼠单克隆抗体[plpc 1] to Myelin PLP -
宿主
Mouse -
特异性
This antibody recognizes myelin proteolipid protein -
经测试应用
适用于: WB, IHC-Fr, IHC-P, ICC/IFmore details
不适用于: Flow Cyt (Intra) -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide:
C-GRGTKF
, corresponding to amino acids 272-277 of Myelin PLP. -
常规说明
This product was switched from a hybridoma to a recombinant production format on 27th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
plpc 1 -
骨髓瘤
Sp2/0 -
同种型
IgG2a -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
靶标
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功能
This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. -
疾病相关
Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. -
序列相似性
Belongs to the myelin proteolipid protein family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5354 Human
- Entrez Gene: 18823 Mouse
- Entrez Gene: 24943 Rat
- Omim: 300401 Human
- SwissProt: P60201 Human
- SwissProt: P60202 Mouse
- SwissProt: P60203 Rat
- Unigene: 1787 Human
see all -
别名
- HLD1 antibody
- Lipophilin antibody
- Major myelin proteolipid protein antibody
see all
图片
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All lanes : Anti-Myelin PLP antibody [plpc 1] (ab9311) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate at 20 µg with NFDM/TBST
Lane 2 : Human liver tissue lysate at 40 µg with NFDM/TBST
Blocking peptides at 5 % per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 20.23 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesSamples are non-boiled as boiling may cause protein aggregates.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9247276).
Negative control: liver (PMID: 2414013).
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.
The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.
The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.
The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.
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Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.
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Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.
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Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1:25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1:1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Myelin PLP with ab9311 at 1/25 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1:1000 dilution (Green). is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1:1000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab9311 被引用在 14 文献中.
- Valcárcel-Hernández V et al. Deficient thyroid hormone transport to the brain leads to impairments in axonal caliber and oligodendroglial development. Neurobiol Dis 162:105567 (2022). PubMed: 34838669
- Cerina M et al. Myelination- and immune-mediated MR-based brain network correlates. J Neuroinflammation 17:186 (2020). PubMed: 32532336
- Göttle P et al. Teriflunomide promotes oligodendroglial differentiation and myelination. J Neuroinflammation 15:76 (2018). WB ; Rat . PubMed: 29534752
- Narayanan V et al. Impairment of frequency-specific responses associated with altered electrical activity patterns in auditory thalamus following focal and general demyelination. Exp Neurol 309:54-66 (2018). PubMed: 30048715
- Mironova YA et al. PI(3,5)P2 biosynthesis regulates oligodendrocyte differentiation by intrinsic and extrinsic mechanisms. Elife 5:N/A (2016). PubMed: 27008179