Anti-Myelin Basic蛋白抗体(ab2404)
Key features and details
- Rabbit polyclonal to Myelin Basic Protein
- Suitable for: IHC-FoFr, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Myelin Basic蛋白抗体
参阅全部 Myelin Basic Protein 一抗 -
描述
兔多克隆抗体to Myelin Basic蛋白 -
宿主
Rabbit -
特异性
This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves. -
经测试应用
适用于: IHC-FoFr, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Full length native protein (purified) corresponding to Human Myelin Basic Protein. Human myelin basic protein isolated from normal human brain.
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阳性对照
- Brain and spinal cord.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Proprietary Purification -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr |
1/1000.
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| IHC-P |
1/20 - 1/40. Antigen retrieval is not essential but may optimise staining.
(ABC method). We suggest an incubation period of 30 minutes at room temperature. |
| 说明 |
|---|
|
IHC-FoFr
1/1000. |
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IHC-P
1/20 - 1/40. Antigen retrieval is not essential but may optimise staining. (ABC method). We suggest an incubation period of 30 minutes at room temperature. |
靶标
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功能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
组织特异性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
疾病相关
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
序列相似性
Belongs to the myelin basic protein family. -
发展阶段
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻译后修饰
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
细胞定位
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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数据库链接
- Entrez Gene: 4155 Human
- Entrez Gene: 24547 Rat
- Omim: 159430 Human
- SwissProt: P02686 Human
- SwissProt: P02688 Rat
- Unigene: 551713 Human
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别名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)Human brain (grey matter) formalin fixed paraffin embedded 4 micron tissue sections. No antigen retrieval was performed. ab2404 (1:40) incubated for 30 min and detected using Mouse/Rabbit PolyVue HRP/ DAB Detection System (a 2-step HRP polymer system for enhanced sensitivity). Tissue was treated with peroxidase block and counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)ab2404 staining Myelin Basic Protein in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry. Staining was detected using DAB. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)Image courtesy of Dr Pezet, CARD Institute, KCL, London, UKab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30
µ Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)Image courtesy of Dr Pezet, CARD institute, KCL, London, UKab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of several images taken with a 40x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
数据表及文件
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Datasheet download
文献 (13)
ab2404 被引用在 13 文献中.
- Alhindi A et al. Terminal Schwann cells at the human neuromuscular junction. Brain Commun 3:fcab081 (2021). PubMed: 33977269
- Oh J et al. Malignant gliomas can be converted to non-proliferating glial cells by treatment with a combination of small molecules. Oncol Rep 41:361-368 (2019). PubMed: 30365111
- Johnson FK et al. Amygdala hyper-connectivity in a mouse model of unpredictable early life stress. Transl Psychiatry 8:49 (2018). PubMed: 29463821
- Armenteros T et al. BMP and WNT signalling cooperate through LEF1 in the neuronal specification of adult hippocampal neural stem and progenitor cells. Sci Rep 8:9241 (2018). PubMed: 29915186
- Bajaj A et al. Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses. J Histochem Cytochem 61:19-30 (2013). PubMed: 23092790
