Anti-Myelin Basic蛋白抗体(ab2404)

Reviews (1)Q&A (3)References (12)

Key features and details

  • Rabbit polyclonal to Myelin Basic Protein
  • Suitable for: IHC-FoFr, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Myelin Basic蛋白抗体
    参阅全部 Myelin Basic Protein 一抗

  • 描述

    兔多克隆抗体to Myelin Basic蛋白

  • 宿主

    Rabbit

  • 特异性

    This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves.

  • 经测试应用

    适用于: IHC-FoFr, IHC-Pmore details

  • 种属反应性

    与反应: Rat, Human

  • 免疫原

    Human myelin basic protein isolated from normal human brain.

  • 阳性对照

    • Brain and spinal cord.

性能

应用

Our Abpromise guarantee covers the use of ab2404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/1000.
IHC-P 1/20 - 1/40. Antigen retrieval is not essential but may optimise staining.

(ABC method). We suggest an incubation period of 30 minutes at room temperature.

靶标

  • 功能

    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

  • 组织特异性

    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

  • 疾病相关

    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).

  • 序列相似性

    Belongs to the myelin basic protein family.

  • 发展阶段

    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.

  • 翻译后修饰

    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

  • 细胞定位

    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt

  • 数据库链接

    • 别名

      • GDB antibody
      • Golli MBP antibody
      • Golli MBP; myelin basic protein antibody
      • Hemopoietic MBP antibody
      • HMBPR antibody
      • HUGO antibody
      • MBP antibody
      • MBP_CAVPO antibody
      • MBP_HUMAN antibody
      • MGC99675 antibody
      • MLD antibody
      • Myelin A1 protein antibody
      • Myelin A1 Protein, basic antibody
      • Myelin basic protein antibody
      • Myelin Deficient antibody
      • Myelin membrane encephalitogenic protein antibody
      • OTTHUMP00000163776 antibody
      • OTTHUMP00000174387 antibody
      • OTTHUMP00000174388 antibody
      • SHI antibody
      • Shiverer antibody
      • SP antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)
      Human brain (grey matter) formalin fixed paraffin embedded 4 micron tissue sections. No antigen retrieval was performed. ab2404 (1:40) incubated for 30 min and detected using Mouse/Rabbit PolyVue HRP/ DAB Detection System (a 2-step HRP polymer system for enhanced sensitivity). Tissue was treated with peroxidase block and counterstained with Hematoxylin.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)
      ab2404 staining Myelin Basic Protein in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry. Staining was detected using DAB.
    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)Image courtesy of Dr Pezet, CARD Institute, KCL, London, UK

      ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30µm thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of 30 images taken with a 20x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.

      Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)Image courtesy of Dr Pezet, CARD institute, KCL, London, UK

      ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of several images taken with a 40x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.

      Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

    文献 (12)

    发表研究结果有使用 ab2404？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2404 被引用在 12 文献中.

    • Oh J  et al. Malignant gliomas can be converted to non-proliferating glial cells by treatment with a combination of small molecules. Oncol Rep 41:361-368 (2019). PubMed: 30365111
    • Johnson FK  et al. Amygdala hyper-connectivity in a mouse model of unpredictable early life stress. Transl Psychiatry 8:49 (2018). PubMed: 29463821
    • Armenteros T  et al. BMP and WNT signalling cooperate through LEF1 in the neuronal specification of adult hippocampal neural stem and progenitor cells. Sci Rep 8:9241 (2018). PubMed: 29915186
    • Bajaj A  et al. Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses. J Histochem Cytochem 61:19-30 (2013). PubMed: 23092790
    • Viertlboeck BC  et al. Chicken immunoregulatory Ig-like receptor families: an overview and expression details on ggTREM-A1. Dev Comp Immunol 41:403-12 (2013). PubMed: 23648646
    View all Publications for this product

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Question

    Hello,

    I am attempting to detect myelination in mouse spinal cords that came from transplanted human cells.

    I would like to use one of your MBP antibodies with immunohistochemistry (preferably immunofluorescence, but peroxidase based DAB reactions will also be acceptable). I want to only detect human MBP, NOT mouse MBP in my mouse spinal cords.

    The following antibodyhttps://www.abcam.com/Myelin-Basic-Protein-antibody-Oligodendrocyte-Marker-ab2404.htmllists that it will label human MBP and cross reacts with rat MBP. Does this mean it will not label mouse MBP? Or has this not been tested?

    Are any of your other MBP antibodies known to recognize human MBP and NOT mouse MBP?
    Thank you very much for your time,

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    Answer

    Thank you for contacting Abcam.

    For ab2404, we list human and rat as reactive species as we guarantee that this antibody will react with those tissues, however we have not tested this antibody against any other tissue. Therefore there is a strong chance that the antibody will bind to mouse tissue, given how similar the sequences are.

    If we know for certain that an antibody will not react to a particular species then we will put that information on the website. If we do not say either way then that means that the antibody is untested in that species and so we do not know how it will react.

    Please let me know if there is anything else I can help you with.

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    Western blot abreview for Anti-Myelin Basic Protein antibody - Oligodendrocyte Marker

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma cells (primary))
    Loading amount
    30 µg
    Specification
    Glioblastoma cells (primary)
    Gel Running Conditions
    Reduced Denaturing (12.5% gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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    Abcam user community

    Verified customer

    提交于 Dec 12 2011

    Question

    DESCRIPTION OF THE PROBLEM No staining. Just hazy in general SAMPLE Adult mouse brain PRIMARY ANTIBODY overnight at 4 degrees in MBP antibody 1:1000, and 1:500 DETECTION METHOD fluorescence microscope POSITIVE AND NEGATIVE CONTROLS USED adult brain ANTIBODY STORAGE CONDITIONS At time of staining, had just reconstituted. Currently at 4 degrees FIXATION OF SAMPLE perfused with paraformaldehyde ANTIGEN RETRIEVAL Sections were double stained for BrdU so sections were first incubated in 2N HCl for 30 min at 37 degrees then immediately in borate buffer for 15 min at room temp. After 3 washes, sections were incubated in .3% hydrogen peroxide for 10 min. PERMEABILIZATION STEP see above BLOCKING CONDITIONS 1 hr room temp in goat serum SECONDARY ANTIBODY 1 hr at room temp in alexafluor 488 1:1000 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? concentration of primary--1:1000 as recommended and 1:500 ADDITIONAL NOTES Have used protocol and secondary many times.

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    Answer

    I'm sorry to hear you are experiencing problems with ab2404. I think the problem might be due to the following: -the antibody may not recognise mouse MBP. The homology of the immunogen with mouse protein was found to only be 71%. It would be useful to therefore try a positive control of human or rat samples - the pretreatment of tissue with HCl may damage the epitope and prevent the antibody from recognizing the protein. I therefore recommend to try without the HCl (and borate) pretreatment. -the perfusion method may not be adequate. The antibody has been tested on PFA-picric acid perfused tissue. The presence of picric acid may be key to a good staining. You do not mention how long you postfix for, this may also affect the epitopes and prevent good recognition by the antibody if the tissue is fixed more than by perfusion. I would finally recommend to add in the blocking buffer and antibody buffer 0.3%v/v triton X100 to help the penetration of the antibody in the tissue. I hope these recommendations will help you,

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    Question

    I would like to stain myelin basic protein in paraffin-embedded brain tissue using chromagen stain. Would this antibody work for this?

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    Answer

    Thank you for your enquiry. Yes, Abcam's ab2404 Myelin Basic Protein antiserum can be applied using immunohistochemistry. We suggest antigen retrieval followed by an incubation period of 30 minutes at room temperature using a dilution range of 1/50 - 1/100 (ABC method). I hope this information helps. Please do not hesitate to contact me should you require any further assistance.

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