Anti-Myelin Basic蛋白抗体(ab2404)
Key features and details
- Rabbit polyclonal to Myelin Basic Protein
- Suitable for: IHC-FoFr, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Myelin Basic蛋白抗体
参阅全部 Myelin Basic Protein 一抗 -
描述
兔多克隆抗体to Myelin Basic蛋白 -
宿主
Rabbit -
特异性
This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves. -
Tested Applications & Species
Application Species IHC-FoFr RatIHC-P Human -
免疫原
Human myelin basic protein isolated from normal human brain.
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阳性对照
- Brain and spinal cord.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading...
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纯度
Proprietary Purification -
克隆
多克隆 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-FoFr |
Rat
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IHC-P |
Human
|
应用 | Ab评论 | 说明 |
---|---|---|
IHC-FoFr |
1/1000.
|
|
IHC-P |
1/20 - 1/40. Antigen retrieval is not essential but may optimise staining.
(ABC method). We suggest an incubation period of 30 minutes at room temperature. |
说明 |
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IHC-FoFr
1/1000. |
IHC-P
1/20 - 1/40. Antigen retrieval is not essential but may optimise staining. (ABC method). We suggest an incubation period of 30 minutes at room temperature. |
靶标
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功能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
组织特异性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
疾病相关
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
序列相似性
Belongs to the myelin basic protein family. -
发展阶段
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻译后修饰
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
细胞定位
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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数据库链接
- Entrez Gene: 4155 Human
- Entrez Gene: 24547 Rat
- Omim: 159430 Human
- SwissProt: P02686 Human
- SwissProt: P02688 Rat
- Unigene: 551713 Human
- Unigene: 63285 Rat
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别名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)Human brain (grey matter) formalin fixed paraffin embedded 4 micron tissue sections. No antigen retrieval was performed. ab2404 (1:40) incubated for 30 min and detected using Mouse/Rabbit PolyVue HRP/ DAB Detection System (a 2-step HRP polymer system for enhanced sensitivity). Tissue was treated with peroxidase block and counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab2404)ab2404 staining Myelin Basic Protein in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry. Staining was detected using DAB.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)Image courtesy of Dr Pezet, CARD Institute, KCL, London, UK
ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30
m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of 30 images taken with a 20x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.µ Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin Basic Protein antibody (ab2404)Image courtesy of Dr Pezet, CARD institute, KCL, London, UK
ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of several images taken with a 40x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
数据表及文件
文献 (12)
ab2404 被引用在 12 文献中.
- Oh J et al. Malignant gliomas can be converted to non-proliferating glial cells by treatment with a combination of small molecules. Oncol Rep 41:361-368 (2019). PubMed: 30365111
- Johnson FK et al. Amygdala hyper-connectivity in a mouse model of unpredictable early life stress. Transl Psychiatry 8:49 (2018). PubMed: 29463821
- Armenteros T et al. BMP and WNT signalling cooperate through LEF1 in the neuronal specification of adult hippocampal neural stem and progenitor cells. Sci Rep 8:9241 (2018). PubMed: 29915186
- Bajaj A et al. Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses. J Histochem Cytochem 61:19-30 (2013). PubMed: 23092790
- Viertlboeck BC et al. Chicken immunoregulatory Ig-like receptor families: an overview and expression details on ggTREM-A1. Dev Comp Immunol 41:403-12 (2013). PubMed: 23648646