为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Adapters Cytoplasmic

Anti-MyD88抗体(ab2064)

  • Datasheet
Reviews (10)Q&A (13)References (84)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-MyD88 antibody (ab2064)
  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
  • Western blot - Anti-MyD88 antibody (ab2064)

Key features and details

  • Rabbit polyclonal to MyD88
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Product image
Recombinant Human MyD88 protein (ab114217)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-MyD88抗体
    参阅全部 MyD88 一抗
  • 描述

    兔多克隆抗体to MyD88
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human MyD88 aa 279-296 (C terminal).
    Sequence:

    CTKSWFWTRLAKALSLP


    (Peptide available as ab7878)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HEK293, HT29, K562, HepG2, A549 and Jurkat whole cell lysate (ab7899). ICC/IF: Jurkat and K562 cells.
  • 常规说明

    MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human MyD88 protein (ab114217)

应用

Our Abpromise guarantee covers the use of ab2064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能

    Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
  • 组织特异性

    Ubiquitous.
  • 疾病相关

    Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
  • 序列相似性

    Contains 1 death domain.
    Contains 1 TIR domain.
  • 结构域

    The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession Q99836 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4615 Human
    • Entrez Gene: 17874 Mouse
    • Entrez Gene: 301059 Rat
    • Omim: 602170 Human
    • SwissProt: Q99836 Human
    • SwissProt: P22366 Mouse
    • SwissProt: Q6Y1S1 Rat
    • Unigene: 82116 Human
    • Unigene: 213003 Mouse
    • Unigene: 37341 Rat
    see all
  • 别名

    • Mutant myeloid differentiation primary response 88 antibody
    • MYD 88 antibody
    • Myd88 antibody
    • MYD88_HUMAN antibody
    • MYD88D antibody
    • Myeloid differentiation marker 88 antibody
    • Myeloid differentiation primary response 88 antibody
    • Myeloid differentiation primary response gene (88) antibody
    • Myeloid differentiation primary response gene 88 antibody
    • Myeloid differentiation primary response gene antibody
    • Myeloid differentiation primary response protein MyD88 antibody
    • OTTHUMP00000161718 antibody
    • OTTHUMP00000208595 antibody
    • OTTHUMP00000209058 antibody
    • OTTHUMP00000209059 antibody
    • OTTHUMP00000209060 antibody
    see all

图片

  • Western blot - Anti-MyD88 antibody (ab2064)
    Western blot - Anti-MyD88 antibody (ab2064)
    All lanes : Anti-MyD88 antibody (ab2064) at 2 µg/ml

    Lane 1 : HEK293 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HT29 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
    Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
    Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed Jurkat cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
    Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
    Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

     

  • Western blot - Anti-MyD88 antibody (ab2064)
    Western blot - Anti-MyD88 antibody (ab2064)This image is courtesy of an anonymous Abreview
    All lanes : Anti-MyD88 antibody (ab2064) at 1 µg/ml

    Lanes 1-2 : Wild type MEFs whole cell lysate
    Lanes 3-4 : MyD88 knockout MEFs whole cell lysate

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 15 seconds

    See Abreview

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (84)

    发表研究结果有使用 ab2064?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2064 被引用在 84 文献中.

    • Che Y  et al. Resveratrol prevents liver damage in MCD-induced steatohepatitis mice by promoting SIGIRR gene transcription. J Nutr Biochem 82:108400 (2020). PubMed: 32438122
    • Chen FX  et al. Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting BAK1. Int J Biol Sci 16:1481-1494 (2020). PubMed: 32226296
    • Wu SJ  et al. Dexmedetomidine Exerted Anti-arrhythmic Effects in Rat With Ischemic Cardiomyopathy via Upregulation of Connexin 43 and Reduction of Fibrosis and Inflammation. Front Physiol 11:33 (2020). PubMed: 32116751
    • Li D  et al. Astragaloside IV protects ATDC5 cells from lipopolysaccharide-caused damage through regulating miR-203/MyD88. Pharm Biol 58:89-97 (2020). PubMed: 31906765
    • Bai C  et al. Effect of High Calorie Diet on Intestinal Flora in LPS-Induced Pneumonia Rats. Sci Rep 10:1701 (2020). PubMed: 32015367
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 23 Abreviews or Q&A

    Western blot abreview for Anti-MyD88 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Specification
    MEFs
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 02 2015

    Western blot abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (macrophages cell line)
    Loading amount
    50 µg
    Specification
    macrophages cell line
    Treatment
    LPS
    Gel Running Conditions
    Reduced Denaturing (10-12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Varkha Agrawal

    Verified customer

    提交于 Sep 10 2012

    Question

    Thanks. It is great if you can send me a hard copy of these inserts..   Regards Shally  : 10/27/11  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2011

    Answer

    Thank you for your reply. I will be happy to mail the datasheets to you. Are you having trouble accessing the datasheets online? I only ask so that I can inform our IT department if there are problems. Could you also please send me the address that you would like the datasheets sent to. Thank you and I look forward to your reply.

    Read More

    Abcam Scientific Support

    回复于 Oct 27 2011

    Question

    Dear Technical, would you please mail us hard copy of thee inserts as we have confusion reading these files.  We hope to be able to receive them tomorrow if you send us today.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2011

    Answer

    The datasheets are accessible from the product pages on the abcam.com website. You could try and print them from there. If you still have difficulty, please let me know and I will mail the datasheets to you.

    Read More

    Abcam Scientific Support

    回复于 Oct 27 2011

    Question

    Dear Technical, we do not have the product insert for the following items. would you please send us copy asap?   Best regard

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    Thank you for contacting Abcam. Please find attached the missing datasheets. If there is anything else I can help you with, please let me kn

    Read More

    Abcam Scientific Support

    回复于 Oct 25 2011

    Question

    I received the vial, but there does not appear to be anything in it.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2011

    Answer

    Thank you for contacting us. I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial which you will receive tomorrow. Please do not hesitate to contact us if you need anything further.

    Read More

    Abcam Scientific Support

    回复于 Oct 11 2011

    Question

    Thank you for your email.I understand that GR 4851-3 & GR 4851-4 antibodies are from the same production batch, but then, I cannot understand why the two aliquots of antibodies gave different specificity results. I attached two pictures from Western blots done with liver samples from mice (20 ug).  Conditions are exactly the same,  in fact the first aliquot of antibody GR 4851-3, was used to establish those conditions in our lab (see below).  However, when we used a second aliquot of the same antibody (GR 4851-4) with the same liver samples that were used before, the results were quite disappointing. We observed many unspecific binging of the primary antibody, which did not diminished with higher dilutions (from 1:250 to 1:500) of the antibody or with longer blocking time (from 1 hour to 2hours) with BSA 2.5% . Western Blot Condition established with the GR 4851-3 aliquot:·       Blocking in BSA 2.5% or 5% in TTBS 1% for 1 hour or 2 hours (Blocking in Milk was also tried)·       Washing 3-4 times for 5 min in TTBS 1%·       1° antibody: different dilution factors have been tested and the one that gave better results was the 1:250 in 10 ml BSA 2.5%. Overnight incubation.·       2° antibody in BSA 2.5% TTBS 1% (unspecific binding given by the 2° antibody alone were not detected) Now that we received another GR 4851-4 aliquot, what are we supposed to do?If we test this new aliquot with the same conditions and with the same samples and have not good results, are we then entitled to be refunded? If this new aliquot works well, will we be refunded for the one that did not give good results? How will we know if further aliquots will be good or not? Considering the high amount of antibody which is required for each WB procedure (about 40 ul) and the total amount of antibody contained in each aliquot 100ul, you understand that we do not have the chance to waste antibody to test if the aliquot is a good one or not. Looking forward to hear from you soon.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 06 2011

    Answer

    Thank you for your reply. I am sorry that you did not have a good experience with a previous vial of ab2064 lot GR 4851-4. I would like to reassure you that if you use this new antibody and do not obtain the desired results, I would be happy to replace this antibody free of charge. I encourage you to test this new vial of ab2064. However, please let me know if the new vial works for you. Also, can you provide an order number for the previous vial of ab2064 you purchased that did not work? I wish you best of luck with your experiments. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Oct 06 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Normal Tonsil)
    Specification
    Human Normal Tonsil
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: DAKO Flex High pH-PTLink
    Permeabilization
    No
    Blocking step
    (agent) for 5 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 27 2011

    Western blot abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    20 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 17 2010

    Western blot abreview for Anti-MyD88 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (smooth muscle cells)
    Loading amount
    15 µg
    Specification
    smooth muscle cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 21 2009

    1-10 of 23 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有