概述

  • 产品名称

    Anti-Myc tag抗体(HRP)
    参阅全部 Myc tag 一抗
  • 描述

    兔多克隆抗体to Myc tag (HRP)
  • 宿主

    Rabbit
  • 偶联物

    HRP
  • 特异性

    In a direct ELISA against 1 ug of C-Myc peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • 经测试应用

    适用于: ELISA, WB, ICCmore details
  • 免疫原

    Synthetic peptide: EQKLISEEDL conjugated to KLH.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support, and conjugated to horseradish peroxidase.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
ICC 1/200 - 1/500.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

文献

This product has been referenced in:

  • Grosse-Holz F  et al. Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in N. benthamiana. Plant Biotechnol J N/A:N/A (2018). Read more (PubMed: 29509983) »
  • Arter M  et al. Regulated Crossing-Over Requires Inactivation of Yen1/GEN1 Resolvase during Meiotic Prophase I. Dev Cell 45:785-800.e6 (2018). Read more (PubMed: 29920281) »
See all 12 Publications for this product

客户评价及客户问答

1-6 of 6 Abreviews or Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Mar 04 2014

Question
Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.


I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thank you for contacting Abcam regarding our Anti-myc antibodies. I have confirmed that yes, ab1326 is the conjugated version of ab9106. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Jun 09 2010

Application
Western blot
Sample
Escherichia coli Recombinant protein (Expressed in DH10B cell)
Loading amount
0.5 µg
Specification
Expressed in DH10B cell
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Mar 17 2010

Answer

No bother at all! Since I hadn't heard back about my last email, the vial was never shipped. I will have a vial, free of charge, shipped out to you straight away. You will receive it at the latest tomorrow. For your record it is order# 41583. Let me know if you have any more questions or concerns.

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