The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000.
1/200. (see Abreview).
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
c-myc tag antibody
Myc Epitope Tag antibody
Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (FITC) (ab1263)Image from Poncet D et al, J Cell Biol. 2006 Sep 25;174(7):985-96. Epub 2006 Sep 18, Fig 3. DOI 10.1083/jcb.200604069
ab1263 staining Myc tag (red) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Zou S et al. A novel actin-binding domain on Slo1 calcium-activated potassium channels is necessary for their expression in the plasma membrane. Mol Pharmacol73:359-68 (2008).
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