Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293T)
Total protein in input
25 µg
Specification
HEK 293T
Immuno-precipitation step
Other - Pre-bound agarose
Other product details
Dilution
1/3
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 50mM HEPES .2% Triton with protease inhi
Western Blot antibody
Name
Non-Abcam antibody was used: Anti-Notch1 C-20
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Additional data
Additional Notes
The dilution step explained: I used a total of 40ul of bead slurry per IP, and diluted it in 120ul of dilution buffer+sample so the total volume of the sample being incubated was ~250ul.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Jonathan Rud
Verified customer
提交于 Dec 28 2009