概述

  • 产品名称
    Anti-Myc tag抗体(Agarose)
    参阅全部 Myc tag 一抗
  • 描述
    山羊多克隆抗体to Myc tag (Agarose)
  • 宿主
    Goat
  • 偶联物
    Agarose
  • 经测试应用
    适用于: IPmore details
    不适用于: ChIP
  • 免疫原

    Full length native protein

    EQKLISEEDL

    (c-myc) (purified): conjugated to KLH.

  • 常规说明

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

性能

应用

Our Abpromise guarantee covers the use of ab1253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.
  • 应用说明
    Is unsuitable for ChIP.
  • 靶标

    • 相关性
      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • 细胞定位
      Nuclear
    • 别名
      • c-myc tag antibody
      • Myc Epitope Tag antibody

    文献

    This product has been referenced in:
    • Cai L  et al. Identification of a genetic interaction between the tumor suppressor EAF2 and the retinoblastoma protein (Rb) signaling pathway in C. elegans and prostate cancer cells. Biochem Biophys Res Commun 447:292-8 (2014). Read more (PubMed: 24727455) »
    • Carballo JA  et al. Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery. PLoS Genet 9:e1003545 (2013). IP . Read more (PubMed: 23825959) »
    See all 8 Publications for this product

    客户评价及客户问答

    1-3 of 3 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK 293T)
    Total protein in input
    25 µg
    Specification
    HEK 293T
    Immuno-precipitation step
    Other - Pre-bound agarose

    Dr. Jonathan Rud

    Verified customer

    提交于 Dec 28 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Immuno-precipitation step
    Other

    Abcam user community

    Verified customer

    提交于 Nov 03 2005

    Answer

    Thank you for your enquiry. You don't need to wash the beads prior to using, and perform the IP at 4C. Also, use 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. Below is a general IP protocol that the originator of this antibody sent that you can use as a guideline. If you have any additional questions, please contact us again. Immunoprecipitation Protocol For use with Antibodies Immobilized on Agarose: A. To microcentrifuge tube add: 1) 15 to 25 ul of slurry of agarose immobilized Ab (3.75 to 6.125 ug Ab) 2)500 ul 2x Stringent IP buffer*: 100 mM Tris-HCl (pH 8.0) 1000 mM Nacl Chelating agents: 2 mM EDTA and/or 2 mM EDTA Detergents: 2 % IGEPAL CA-630 (replacement for NP40) 0.2 % SDS 2 % Deoxycholate Protease Inhibitors: 20 ug/ml Aprotinin 20 ug/ml Leupeptin 2 mM PMSF or 10 ug/ml Pepstatin A or 8 mM AEBSF Phosphatase Inhibitors – If Necessary: 200 mM NaF 2 mM Na3VO4 50 mM ?-Glycerophosphate 3)Cell lysate (0.1 to 1.0 mg total protein) 4)diH2O to a total volume of 1 ml B. Vortex and incubate for between 1 and 24 h at 4 deg. Turning tubes on a rotating platform wheel allows continuous mixing. C. Centrifuge (16,000 x g, 3 to 5 min), aspirate and discard supernatant D. Wash pellet in 1x IP buffer 3 to 6 times. Washing consists of resuspending pellet, centrifuging (16,000 x g, 3 to 5 min), aspirating and discarding supernatant. E. After last wash, resuspend pellet in 30 ul 2x Electrophoresis Sample Buffer, boil 5 min. F. Centrifuge, load supernatant onto SDS-PAGE gel, electroblot onto PVDF membrane and perform Western Blot Analysis. *Buffers for Immunoprecipitation Assays For analysis of a single protein without associated proteins, the use of high ionic strength, strong detergents, and rigorous washing of the immunoprecipitate are recommended. One suitable buffer is the Strigent IP buffer outlined above. For co-precipitation of associated proteins, the use of near physiological ionic strength and gentle detergents is recommended. One suitable buffer is the Lenient IP buffer outlined below. The buffers outlined represent two extremes. The optimal buffer for ones given application must be empirically defined. This buffer may be of intermediate ionic strength and/or detergent composition. 1x Lenient IP buffer: 50 mM Tris-HCl (pH 8.0) 100 mM Nacl Chelating agents: 1 mM EDTA and/or 1 mM EGTA Detergents: 0.5 % IGEPAL CA-630 (replacement for Nonidet P40) Protease Inhibitors: 10 ug/ml Aprotinin 10 ug/ml Leupeptin 1 mM PMSF or 5 ug/ml Pepstatin A or 4 mM AEBSF Phosphatase Inhibitors – If Necessary: 100 mM NaF 1 mM Na3VO4 50 mM ?-Glycerophosphate

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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