概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • 纯度
    IgY fraction
  • 纯化说明
    Antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a SulfoLink® (Pierce) column conjugated with the peptide used for immunizations, and then dialyzed against PBS. Filter-sterilized (0.2 µm).
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 19679075
WB 1/500. Can be blocked with Human c-Myc peptide (ab13837).
IP 1/250.
ICC 1/250.

靶标

  • 相关性
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位
    Nuclear
  • 别名
    • c-myc tag antibody
    • Myc Epitope Tag antibody

文献

This product has been referenced in:
  • Czarna A  et al. Single-cell analysis of the fate of c-kit-positive bone marrow cells. NPJ Regen Med 2:27 (2017). Read more (PubMed: 29302361) »
  • Rutkowska-Wlodarczyk I  et al. A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with Go proteins. J Neurosci 35:5171-9 (2015). Read more (PubMed: 25834043) »

See all 4 Publications for this product

发表研究结果有使用 ab172？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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8 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Myc tag antibody

 Average
Abreviews
Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Username

Abcam user community

Verified customer

提交于 May 16 2014

Hi Jennifer, Thank you for looking into this for me-it would be great to try another batch. Thanks, kathleen

A replacement vial is being sent to you and you should receive it tomorrow. For your record it is order# 39089. I did receive some information from the antibody's originator which I hope will also help you out concerning cell fixation. "We have fou...

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I have tested this antibody again in a cell line expressing a myc-tagged protein, well recognized by 9E10. It again did not work for me. Do you have any suggestions (I filled out the technical inquiry form at length).

Thank you for your patience. I was away on vacation and could not get back to you earlier. We have had no other complaints regarding this antibody. You're getting absolutely no signal? Are you doing antigen retrieval? You stated that your cells are ...

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I think that my purchasing agent was not clear. The antibody has not worked for me yet, but my current complaint is that Federal Express did not handle the shipping properly. It was shipped by you Fed Ex overnight but sat at Fed Ex for an additional da...

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I looked at the tracking for Fed Ex and it states that the package was delivered on Feb 25 at 11am and signed for by L. Kim. Although the antibody arrived a day late, it should not affect the antibody (it should have still been cold). If you continue t...

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Thanks for the info. I was wondering if you guys might have the goat anti-chicken IgY that i need. If so, could you please send me some.

We don't have an unlabeled goat anti-chicken IgY antibody (all of ours are conjugated). Aves lab has one: Cat.# U-1010. If you have any more questions, please contact us again.

Should protein A, G or L be used for an immunoprecipitation with this antibody (ab172)?

I contacted the supplier of ab172, and they provided me with the following information. Chicken IgY's lack an Fc domain in the "nu" heavy chain which where proteins A and G normally bind. Consequently, protein A/G-Agarose cannot be used directly t...

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Dear Aves Labs, I would like to know if the chicken polyclonal anti-myc antibody has even been used for immnofluoresence with yeast. Thanks, Colin MacDiarmid.

I'm afraid that I don't know of anyone who has used the c-myc antibodies for immunofluorescence in yeast, either. Sorry.

Dear Supplier, I would like to know if the chicken polyclonal c-myc epitope tag has been successfully used for indirect immunofluorescence in Drosophila melanogaster. Thank you for your help, Kathleen Sullivan

Dear Kathleen, has Aves replied to this yet? If not their direct email is: info@aveslab.com

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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