Application
ChIP
Sample
Human Cell lysate - whole cell (skin fibroblasts)
Specification
skin fibroblasts
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
NPM1 cMYC binding site
Negative control
IgG pull-down
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Triton, 150mM NaCl, 0.1% SDS in TE
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提交于 Jul 15 2011