Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Sample
Human Cell lysate - whole cell (HEK 293)
Specification
HEK 293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/5000
Detection
Detection method
Licor
Bands
Specific: 52 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 26 2014