Application
Immunocytochemistry/ Immunofluorescence
Sample
Saccharomyces cerevisiae Cell (BY4147 background)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
BY4147 background
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/1000
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used:
Additional data
Additional Notes
Protein that was tagged with 13xMYC were used for Immunocytochemistry.
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提交于 Jun 01 2011