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I would like to use the myc tag ab 9E10 for immunoprecipitation. Can I use protein A to IP which I have already got or it is better to use Protein G which I have read has a higher affinity for IgG1.
Asked on Nov 15 2004
You are right, not all the classes or subclasses of immunoglobulin from all species bind tightly to Protein A. For those that do not insolubilized Protein G, Protein A/G or a second antibody is used to allow precipitation. As a general rule, for mouse IgG1 (this antibody is raised in mouse and the subclasses is IgG1) Protein G would be a better choice. However, you may also be able to use Protein A successfully (although the affinity is lower), so just have a go since you already have this material in your lab. We would like to draw your attention to the published reviews on-line, particularly the IP-related ones. One particular customer has reported that Protein G worked nicely. We hope this information will be useful for you.
回复于 Nov 16 2004