Anti-Myc tag抗体[9E10] - BSA and Azide free (ab264603)

概述

  • 产品名称

    Anti-Myc tag抗体[9E10] - BSA and Azide free
    参阅全部 Myc tag 一抗
  • 描述

    小鼠单克隆抗体[9E10] to Myc tag - BSA and Azide free
  • 宿主

    Mouse
  • 特异性

    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PubMed ID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression.

  • 经测试应用

    适用于: ICC/IF, ICC, Flow Cyt, WB, IP, ELISA, IHC-P, IHC-Fr, Purificationmore details
  • 免疫原

    Synthetic peptide corresponding to Human Myc tag aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.

  • 表位

    aa 410-419 of human Myc.
  • 阳性对照

    • Myc tagged proteins and myc tag expressing cells.
  • 常规说明

    ab264603 is a PBS only version of ab32.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    Constituent: PBS
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 克隆

    单克隆
  • 克隆编号

    9E10
  • 骨髓瘤

    Sp2/0
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab264603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
ICC 1/2000.

See Abreviews.

Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/1000.
IP Use at 6 µg/mg of lysate.
ELISA Use at an assay dependent concentration.
IHC-P 1/250 - 1/500.
IHC-Fr 1/1000. See Abreviews.
Purification Use at an assay dependent concentration.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

图片

  • Anti-Myc tag antibody [9E10] - ChIP Grade (ab32) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, Azide and Arginine (ab32).

    Lysate from E. coli recombinantly expressing 11 commonly used tags including myc tag.

文献

ab264603 has not yet been referenced specifically in any publications.

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