概述

  • 产品名称

    Anti-Myc tag抗体[9E10]
    参阅全部 Myc tag 一抗
  • 描述

    大鼠单克隆抗体[9E10] to Myc tag
  • 宿主

    Rat
  • 特异性

    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
  • 经测试应用

    适用于: IHC-Fr, IHC-P, ICC/IF, IP, WBmore details
  • 免疫原

    Synthetic peptide corresponding to Human c-Myc aa 408-439 (C terminal).
    Sequence:

    AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA


    Database link: P01106

  • 表位

    Epitope located at aa 410-419; EQKLISEEDL
  • 常规说明

    ab206486 is a recombinant antibody that comprises the variable regions from the 9E10 hybridoma within a rat IgG1 scaffold.

性能

应用

Our Abpromise guarantee covers the use of ab206486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

图片

  • All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 2 µg/ml

    Lane 1 : HEK-293 spiked with 5 ng multitag protein
    Lane 2 : HEK-293 unspiked with 5 ng multitag protein
    Lane 3 : 25 ng purified multitag protein

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 48 kDa

  • All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 1 µg/ml

    Lane 1 : Multitag protein at 0.025 µg
    Lane 2 : Multitag protein at 0.01 µg
    Lane 3 : Multitag protein at 0.005 µg

    Predicted band size: 48 kDa

文献

This product has been referenced in:

  • Compte M  et al. A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity. Nat Commun 9:4809 (2018). Read more (PubMed: 30442944) »
  • Ferro M  et al. Functional mapping of brain synapses by the enriching activity-marker SynaptoZip. Nat Commun 8:1229 (2017). Read more (PubMed: 29089485) »
See all 5 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton 5 minutes
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

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提交于 Jan 25 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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