重组Anti-Myc tag抗体[9E10] (ab206486)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [9E10] to Myc tag
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IP, WB
概述
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产品名称
Anti-Myc tag抗体[9E10]
参阅全部 Myc tag 一抗 -
描述
大鼠单克隆抗体[9E10] to Myc tag -
宿主
Rat -
特异性
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
经测试应用
适用于: IHC-Fr, IHC-P, ICC/IF, IP, WBmore details -
免疫原
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal).
Database link: P01106 -
表位
Epitope located at aa 410-419; EQKLISEEDL -
常规说明
ab206486 is a recombinant antibody that comprises the variable regions from the 9E10 hybridoma within a rat IgG1 scaffold.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
9E10 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- HRP Anti-Myc tag antibody [9E10] (ab62928)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
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Assay kits
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab206486于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IP | (1) |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. |
靶标
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相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
图片
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All lanes : Anti-Myc tag antibody [9E10] (ab32) at 1/200 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDaFalse colour image of Western blot: Anti-Myc tag antibody [9E10] staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 2 µg/ml
Lane 1 : HEK-293 spiked with 5 ng multitag protein
Lane 2 : HEK-293 unspiked with 5 ng multitag protein
Lane 3 : 25 ng purified multitag protein
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDa -
All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 1 µg/ml
Lane 1 : Multitag protein at 0.025 µg
Lane 2 : Multitag protein at 0.01 µg
Lane 3 : Multitag protein at 0.005 µg
Predicted band size: 48 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab206486 被引用在 15 文献中.
- Xin S et al. MS4A15 drives ferroptosis resistance through calcium-restricted lipid remodeling. Cell Death Differ 29:670-686 (2022). PubMed: 34663908
- Tapia-Galisteo A et al. Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer. Oncoimmunology 11:2034355 (2022). PubMed: 35154908
- D'Amico G et al. ERG activity is regulated by endothelial FAK coupling with TRIM25/USP9x in vascular patterning. Development 149:N/A (2022). PubMed: 35723257
- Guo Q et al. ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage. Cell Cycle 20:1692-1707 (2021). PubMed: 34334113
- Liu Y et al. Ginsenoside Rh2 stimulates the production of mitochondrial reactive oxygen species and induces apoptosis of cervical cancer cells by inhibiting mitochondrial electron transfer chain complex. Mol Med Rep 24:N/A (2021). PubMed: 34713297