重组Anti-MX1抗体[EPR19967] (ab207414)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19967] to MX1
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-MX1抗体[EPR19967]
参阅全部 MX1 一抗 -
描述
兔单克隆抗体[EPR19967] to MX1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IP, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Daudi treated with 20U/ml IFN alpha 1 for 24 hours whole cell lysate; Human spleen and tonsil lysates; Untreated Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate ICC/IF: Daudi cells treated with 20U/ml IFN alpha 1 for 24 hours. IP: Daudi treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate Flow Cyt (intra): Daudi cells
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19967 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab207414于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP |
1/40 - 1/1000.
|
|
ICC/IF |
1/250.
|
|
WB |
1/1000 - 1/2000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
|
说明 |
---|
Flow Cyt (Intra)
1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/40 - 1/1000. |
ICC/IF
1/250. |
WB
1/1000 - 1/2000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). |
靶标
-
功能
May regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus. -
序列相似性
Belongs to the dynamin family.
Contains 1 GED domain. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 4599 Human
- Omim: 147150 Human
- SwissProt: P20591 Human
- Unigene: 517307 Human
-
别名
- Human interferon regulated resistance GTP binding protein MXA antibody
- IFI 78 antibody
- IFI 78K antibody
see all
图片
-
All lanes : Anti-MX1 antibody [EPR19967] (ab207414) at 1/2000 dilution
Lane 1 : Untreated Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 76 kDa
Observed band size: 76 kDaBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBSTExposure time: 3.25 seconds
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (Human Burkitt's lymphoma cell line) cells labeling MX1 with ab207414 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing negative staining on Daudi cell line. Expression was induced and showed cytoplasmic staining after cells were treated with IFN alpha 1 (20 U/ml) for 24h. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
All lanes : Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution
Lanes 1-2 : Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207414 in Daudi treated with 20 U/ml IFN alpha 1 for 24 hours whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP detection reagent (HRP))
Observed band size: 76 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) treated with 20U/mL IFN alpha 1 for 24h cells labeling MX1 with purified ab207414 at 1/500 dilution (1µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). Untreated control (Green).
-
All lanes : Anti-MX1 antibody [EPR19967] (ab207414) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lane 2: 1 minute.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (6)
ab207414 被引用在 6 文献中.
- McFadden MJ et al. Post-transcriptional regulation of antiviral gene expression by N6-methyladenosine. Cell Rep 34:108798 (2021). PubMed: 33657363
- Song JY et al. Epigallocatechin-3-gallate Can Prevent Type 2 Human Papillomavirus E7 from Suppressing Interferon-Stimulated Genes. Int J Mol Sci 22:N/A (2021). PubMed: 33670861
- Williams GD et al. The mRNA Cap 2'-O-Methyltransferase CMTR1 Regulates the Expression of Certain Interferon-Stimulated Genes. mSphere 5:N/A (2020). PubMed: 32404510
- Blanco-Melo D et al. Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19. Cell 181:1036-1045.e9 (2020). PubMed: 32416070
- Bayne RS et al. MAP3K7 and CHD1 Are Novel Mediators of Resistance to Oncolytic Vesicular Stomatitis Virus in Prostate Cancer Cells. Mol Ther Oncolytics 17:496-507 (2020). PubMed: 32529027
- Kang M et al. MicroRNA-211 promotes non-small-cell lung cancer proliferation and invasion by targeting MxA. Onco Targets Ther 10:5667-5675 (2017). PubMed: 29238200