Anti-MVP抗体(ab97311)
Key features and details
- Rabbit polyclonal to MVP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MVP抗体
参阅全部 MVP 一抗 -
描述
兔多克隆抗体to MVP -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Cat
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免疫原
Recombinant fragment corresponding to Human MVP aa 158-350.
Sequence:EVV EIIQATIIRQ NQALRLRARK ECWDRDGKER VTGEEWLVTT VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ARRNFRDFRG VSRRTGEEWL VTVQDTEAHV PDVHEEVLGV VPITTLGPHN YCVILDPVGP DGKNQLGQKR VVKGEKSFFL QPGEQLEQGI QDVYVLSEQQ GLLLRALQPL EEGEDEEKVS
Database link: Q14764 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
|
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| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 说明 |
|---|
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ICC/IF
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa. |
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IHC-P
1/100 - 1/1000. |
靶标
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功能
Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases. -
组织特异性
Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells. -
序列相似性
Contains 9 MVP (vault) repeats. -
结构域
MVP 3 mediates interaction with PTEN.
MVP 4 mediates interaction with PARP4. -
翻译后修饰
Phosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11. -
细胞定位
Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 9961 Human
- Entrez Gene: 78388 Mouse
- Entrez Gene: 64681 Rat
- Omim: 605088 Human
- SwissProt: Q14764 Human
- SwissProt: Q9EQK5 Mouse
- SwissProt: Q62667 Rat
- Unigene: 632177 Human
see all -
别名
- LRP antibody
- Lung resistance related protein antibody
- Lung resistance-related protein antibody
see all
图片
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Western blot - Anti-MVP antibody (ab97311)All lanes : Anti-MVP antibody (ab97311) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : MVP Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 99 kDa -
Western blot - Anti-MVP antibody (ab97311)Anti-MVP antibody (ab97311) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 99 kDa
7.5% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody (ab97311)Immunohistochemical analysis of paraffin-embedded A549 xenograft using ab97311 at 1/500 dilution.
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Western blot - Anti-MVP antibody (ab97311)All lanes : Anti-MVP antibody (ab97311) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : BCL-1 whole cell lysate
Lane 5 : Raw264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 99 kDa7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody (ab97311)A431 cells stained for MVP/LRP (green) using ab97311 at 1/200 dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody (ab97311)Paraffin embedded mouse colon tissue stained for MVP/LRP using ab97311 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody (ab97311)Paraffin embedded human breast carcinoma tissue stained for MVP/LRP using ab97311 at 1/500 dilution in immunohistochemical analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97311 被引用在 2 文献中.
- Shi L et al. Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J. BMC Cancer 17:138 (2017). WB . PubMed: 28202053
- O'Dwyer D et al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr ; Human . PubMed: 22125622