Anti-Munc18-1抗体(ab3451)
Key features and details
- Rabbit polyclonal to Munc18-1
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Munc18-1抗体
参阅全部 Munc18-1 一抗 -
描述
兔多克隆抗体to Munc18-1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human Munc18-1 aa 58-70.
Sequence:VEDINKRREPIPS
(Peptide available asab4982) -
阳性对照
- WB: Mouse and rat brain tissue lysates ICC: U-87 MG cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa.
By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract. |
| ICC |
Use a concentration of 2 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa. By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract. |
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ICC
Use a concentration of 2 µg/ml. |
靶标
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功能
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
组织特异性
Brain and spinal cord. Highly enriched in axons. -
疾病相关
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
序列相似性
Belongs to the STXBP/unc-18/SEC1 family. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 404293 Chicken
- Entrez Gene: 282378 Cow
- Entrez Gene: 6812 Human
- Entrez Gene: 20910 Mouse
- Entrez Gene: 25558 Rat
- Omim: 602926 Human
- SwissProt: Q6R748 Chicken
- SwissProt: P61763 Cow
see all -
别名
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
图片
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Western blot - Anti-Munc18-1 antibody (ab3451)Anti-Munc18-1 antibody (ab3451) at 2 µg/ml + Mouse brain tissue lysate at 30 µg
Secondary
Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution
Observed band size: 67 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-Munc18-1 antibody (ab3451)Immunocytochemistry analysis of 70% confluent log phase U-87 MG cells labeling Munc18-1 with ab3451 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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Western blot - Anti-Munc18-1 antibody (ab3451)All lanes : Anti-Munc18-1 antibody (ab3451) at 2 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution
Observed band size: 67 kDa why is the actual band size different from the predicted?Detected by chemiluminescence
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Western blot - Anti-Munc18-1 antibody (ab3451)This image is courtesy of an anonymous AbreviewAnti-Munc18-1 antibody (ab3451) at 1/5000 dilution + Mouse hippocampal homogenate at 5 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 67 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-Munc18-1 antibody (ab3451)Western blot of Munc-18 in rat brain homogenate immunoprecipitated with ab3451.
1. Extract only.
2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of ab3451, respectively.
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab3451 被引用在 7 文献中.
- Chen W et al. Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy. Elife 9:N/A (2020). PubMed: 32073399
- Henderson BW et al. Rho-associated protein kinase 1 (ROCK1) is increased in Alzheimer's disease and ROCK1 depletion reduces amyloid-ß levels in brain. J Neurochem 138:525-31 (2016). PubMed: 27246255
- Wagnon JL et al. CELF4 regulates translation and local abundance of a vast set of mRNAs, including genes associated with regulation of synaptic function. PLoS Genet 8:e1003067 (2012). WB, IHC-FoFr ; Mouse . PubMed: 23209433
- Kim K et al. Munc18b is an essential gene in mice whose expression is limiting for secretion by airway epithelial and mast cells. Biochem J 446:383-94 (2012). PubMed: 22694344
- Ramos C et al. Native pre-synaptic metabotropic glutamate receptor 4 (mGluR4)interacts with exocytosis proteins in rat cerebellum. J Biol Chem : (2012). WB . PubMed: 22528491
- Bajor M et al. Synaptic cell adhesion molecule-2 and collapsin response mediator protein-2 are novel members of the matrix metalloproteinase-9 degradome. J Neurochem 122:775-88 (2012). PubMed: 22694054
- Procino G et al. AQP2 exocytosis in the renal collecting duct -- involvement of SNARE isoforms and the regulatory role of Munc18b. J Cell Sci 121:2097-106 (2008). WB, ICC/IF ; Mouse . PubMed: 18505797
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