Anti-Munc18-1抗体(ab3451)
Key features and details
- Rabbit polyclonal to Munc18-1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Munc18-1抗体
参阅全部 Munc18-1 一抗 -
描述
兔多克隆抗体to Munc18-1 -
宿主
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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免疫原
Synthetic peptide corresponding to Human Munc18-1 aa 58-70.
Sequence:VEDINKRREPIPS
(Peptide available asab4982) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| WB |
Rat
|
| All applications |
Chicken
Cow
Human
Pig
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa.
By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract. |
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa. By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract. |
靶标
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功能
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
组织特异性
Brain and spinal cord. Highly enriched in axons. -
疾病相关
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
序列相似性
Belongs to the STXBP/unc-18/SEC1 family. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 404293 Chicken
- Entrez Gene: 282378 Cow
- Entrez Gene: 6812 Human
- Entrez Gene: 25558 Rat
- Omim: 602926 Human
- SwissProt: Q6R748 Chicken
- SwissProt: P61763 Cow
- SwissProt: P61764 Human
see all -
别名
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
图片
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Western blot - Anti-Munc18-1 antibody (ab3451)This image is courtesy of an anonymous AbreviewAnti-Munc18-1 antibody (ab3451) at 1/5000 dilution + Mouse hippocampal homogenate at 5 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 67 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-Munc18-1 antibody (ab3451)Western blot of Munc-18 in rat brain homogenate immunoprecipitated with ab3451.
1. Extract only.
2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of ab3451, respectively.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab3451 被引用在 7 文献中.
- Chen W et al. Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy. Elife 9:N/A (2020). PubMed: 32073399
- Henderson BW et al. Rho-associated protein kinase 1 (ROCK1) is increased in Alzheimer's disease and ROCK1 depletion reduces amyloid-ß levels in brain. J Neurochem 138:525-31 (2016). PubMed: 27246255
- Wagnon JL et al. CELF4 regulates translation and local abundance of a vast set of mRNAs, including genes associated with regulation of synaptic function. PLoS Genet 8:e1003067 (2012). WB, IHC-FoFr ; Mouse . PubMed: 23209433
- Kim K et al. Munc18b is an essential gene in mice whose expression is limiting for secretion by airway epithelial and mast cells. Biochem J 446:383-94 (2012). PubMed: 22694344
- Ramos C et al. Native pre-synaptic metabotropic glutamate receptor 4 (mGluR4)interacts with exocytosis proteins in rat cerebellum. J Biol Chem : (2012). WB . PubMed: 22528491
- Bajor M et al. Synaptic cell adhesion molecule-2 and collapsin response mediator protein-2 are novel members of the matrix metalloproteinase-9 degradome. J Neurochem 122:775-88 (2012). PubMed: 22694054
- Procino G et al. AQP2 exocytosis in the renal collecting duct -- involvement of SNARE isoforms and the regulatory role of Munc18b. J Cell Sci 121:2097-106 (2008). WB, ICC/IF ; Mouse . PubMed: 18505797