Anti-Munc18-1抗体(ab155020)
Key features and details
- Rabbit polyclonal to Munc18-1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Munc18-1抗体
参阅全部 Munc18-1 一抗 -
描述
兔多克隆抗体to Munc18-1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Xenopus laevis -
免疫原
Recombinant fragment corresponding to a region within amino acids 354-574 of Human Munc18.
-
阳性对照
- 293T, A431, Jurkat, Raji and mouse brain lysates; A431 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155020于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
-
功能
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
组织特异性
Brain and spinal cord. Highly enriched in axons. -
疾病相关
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
序列相似性
Belongs to the STXBP/unc-18/SEC1 family. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 404293 Chicken
- Entrez Gene: 282378 Cow
- Entrez Gene: 6812 Human
- Entrez Gene: 20910 Mouse
- Entrez Gene: 25558 Rat
- Entrez Gene: 399128 Xenopus laevis
- Omim: 602926 Human
- SwissProt: Q6R748 Chicken
see all -
别名
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
图片
-
All lanes : Anti-Munc18-1 antibody (ab155020) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE -
Anti-Munc18-1 antibody (ab155020) at 1/10000 dilution + mouse brain whole cell lysate at 20 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Confocal immunofluorescent analysis of methanol-fixed A431 cells labeling Munc18 with ab155020 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody at 1/2000 dilution (red).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab155020 尚未被引用在任何文献中。