Anti-Mucin 5AC抗体[CLH2] (ab77576)
Key features and details
- Mouse monoclonal [CLH2] to Mucin 5AC
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Mucin 5AC抗体[CLH2]
参阅全部 Mucin 5AC 一抗 -
描述
小鼠单克隆抗体[CLH2] to Mucin 5AC -
宿主
Mouse -
特异性
ab77576 is specific for the MUC5AC tandem repeat of human mucin. No cross reactivity with other peptides from MUC2, 3, 6 or 7 The antibody recognises glycosylated as well as unglycosylated MUC5AC. -
经测试应用
适用于: IHC-Fr, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma.
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常规说明
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
CLH2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77576于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. -
组织特异性
Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 4 VWFC domains.
Contains 4 VWFD domains. -
结构域
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
翻译后修饰
C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis.
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4586 Human
- Omim: 158373 Human
- SwissProt: P98088 Human
- Unigene: 534332 Human
- Unigene: 558950 Human
- Unigene: 703588 Human
- Unigene: 703728 Human
- Unigene: 721515 Human
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别名
- Apomucin antibody
- Apomucin Major Airway Glycoprotein antibody
- Gastric mucin antibody
see all
图片
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ab77576 staining of foveolar epithelium of human gastric mucosa using IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex. Colour Development by DAB. Hematoxylin counterstained.
This image was generated using the ascites version of the product.
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ab77576 staining of foveolar epithelium of human gastric mucosa by IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex.
This image was generated using the ascites version of the product.
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Overlay histogram showing A549 cells stained with ab77576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab77576 被引用在 6 文献中.
- Uehara K et al. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. Stem Cell Reports 17:820-834 (2022). PubMed: 35245440
- Beisl J et al. Assessing Mixture Effects of Cereulide and Deoxynivalenol on Intestinal Barrier Integrity and Uptake in Differentiated Human Caco-2 Cells. Toxins (Basel) 13:N/A (2021). PubMed: 33806705
- Zhang J et al. Evaluation of serum MUC5AC in combination with CA19-9 for the diagnosis of pancreatic cancer. World J Surg Oncol 18:31 (2020). PubMed: 32028958
- Lee HY et al. Platycodi Radix and its active compounds ameliorate against house dust mite-induced allergic airway inflammation and ER stress and ROS by enhancing anti-oxidation. Food Chem Toxicol 123:412-423 (2019). PubMed: 30399386
- Sim HJ et al. Xenopus: An alternative model system for identifying muco-active agents. PLoS One 13:e0193310 (2018). PubMed: 29470529
- Kaur S et al. A Combination of MUC5AC and CA19-9 Improves the Diagnosis of Pancreatic Cancer: A Multicenter Study. Am J Gastroenterol 112:172-183 (2017). PubMed: 27845339