概述

  • 产品名称
    Anti-Mucin 5AC抗体[45M1] - BSA and Azide free
    参阅全部 Mucin 5AC 一抗
  • 描述
    小鼠单克隆抗体[45M1] to Mucin 5AC - BSA and Azide free
  • 宿主
    Mouse
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cat, Human, Pig, Monkey, Hedgehog
    不与反应: Cow
  • 免疫原

    Tissue, cells or virus corresponding to Human Mucin 5AC. (M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient).

  • 表位
    Located in the C-terminal cysteine rich part of the peptide core of MUC5AC.
  • 阳性对照
    • Human stomach, Human gastric carcinoma and Rat stomach tissues.

性能

应用

Our Abpromise guarantee covers the use of ab212636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

(in 0.1 ml).

IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

靶标

  • 相关性
    Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.
  • 细胞定位
    Secreted; Cytoplasmic and cell surface
  • 数据库链接
  • 别名
    • Apomucin antibody
    • Apomucin Major Airway Glycoprotein antibody
    • Gastric mucin antibody
    • LeB antibody
    • Lewis B blood group antigen antibody
    • Major Airway Glycoprotein antibody
    • MUC 5 antibody
    • MUC5 antibody
    • MUC5AC antibody
    • Mucin 5 subtype AC tracheobronchial antibody
    • Mucin 5 Subtypes A And C antibody
    • Mucin 5 subtypes A and C tracheobronchial/gastric antibody
    • Mucin 5, tracheobronchial/gastric antibody
    • Mucin 5AC antibody
    • Mucin 5AC oligomeric mucus/gel forming antibody
    • Mucin 5AC, oligomeric mucus/gel forming pseudogene antibody
    • Mucin antibody
    • TBM antibody
    • Tracheobronchial Mucin (TBM) antibody
    • Tracheobronchial mucin antibody
    see all

图片

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling Mucin 5AC with ab212636 at 2 μg/ml dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling Mucin 5AC with ab212636 at 2 μg/ml dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat stomach tissue labeling Mucin 5AC with ab212636 at 2 μg/ml dilution.

文献

ab212636 has not yet been referenced specifically in any publications.

客户评价及客户问答

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1-3 of 3 Abreviews

Application
ELISA
Sample
Human Purified protein (gastric mucin)
Specification
gastric mucin
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type
Indirect

Abcam user community

Verified customer

提交于 Jun 15 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - Triton X-100
Specification
Small intestine
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Dr. Egi Kardia

Verified customer

提交于 Dec 07 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Corneal goblet cells)
Antigen retrieval step
None
Permeabilization
Yes - Triton 0,3% - 5 minutesr
Specification
Corneal goblet cells
Blocking step
Normal Goat Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Aug 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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